Table 1.
Cross-linked peptide pairs between P450 11B1/11B2 and Adx (A & B molecules)
| Index | Charge | PP* | PP2 | PPtag | m/z | MW(obs) | MW | CYP11B1** | CYP11B2** | Adx** | Modification*** |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3433 | 4 | 37.3 | 24.1 | 1.7 | 348.1758 | 1389.6815 | 1389.6951 | HPQKAT | TVADARQ(B) | PyrQ | |
| 4433 | 3 | 6.7 | 14.0 | 1.8 | 550.2820 | 1648.8314 | 1648.8180 | KAALKETL | EENDML(A) | AceB, DANQ | |
| 4924 | 3 | 16.9 | 14.2 | 3.7 | 402.2232 | 1204.6552 | 1204.6606 | KAALK | DMLDLA(A) | OxiM | |
| 6267 | 4 | 18.3 | 9.6 | 1.4 | 767.8810 | 3068.5023 | 3068.4841 | ASISEHPQKATTEL | DNMTVRVPETVADA(B) | AceB, OxiM | |
| 6457 | 4 | 35.1 | 14.3 | 2.6 | 343.9506 | 1372.7805 | 1372.7756 | TELPLLKA | MLDL(A) | OxiM | |
| 6990 | 4 | 28.1 | 14.0 | 4.5 | 351.9259 | 1404.6817 | 1404.6866 | ISEHPQKA | DARQ(B) | PyrQ, AceB | |
| 9782 | 2 | 10.9 | 12.6 | 2.1 | 903.9162 | 1806.8251 | 1806.8426 | RAALKET | EENDMLDL(A) | DANQ, OxiM, AceB |
*
The quality of a peptide match is mainly evaluated by three statistical scores: pp, pp2, pptag. A peptide match with max (pp,pp2) > 3.7 and pptag > 1.3 is considered to be significant with p value < 0.05.
**
The cross-linked residues are underlined.
***
Variable modifications: deamidation of NQ (DANQ), pyro-Glu from Q (PyrQ), oxidation of M (OxiM), and acetylation of N-term (AceB)