Figure 6.

TNEA treatment promoted the formation of autolysosomes in 5XFAD mice brains. Representative fluorescent images of LC3B (green) and CTSD (red) in the prefrontal cortex (PFC) (A) and hippocampal (HI) CA1 (B) of mice from wild-type (WT), 5XFAD and 5XFAD+TNEA groups. Original magnification: 60×, scale bar: 50 μm. Corresponding zoom-in images (scale bar: 25 μm) were processed using ImageJ to demonstrate the colocalization. The area of LC3B-positive CTSD was quantified as mean ± SEM (male, n = 3 to 5) and analyzed by one‐way ANOVA. *p < 0.05, **p < 0 .01 and ***p < 0.001 vs. 5XFAD group