Figure 3.
Loss of IFN-I signaling in HNSCC cells promotes cancer stemness. (a-b) EV control and shIfnar1 MOC2-E6/E7 cells were stained using the ALDEFLUOR Assay Kit and analyzed via flow cytometry. Immunoblots were performed to compare the expression levels of the indicated cancer stemness markers in EV control and shIfnar1-MOC2-E6/E7 cells (c) as well as EV control and shIfnar1-NOOC1 cells (d). Experiments were performed twice. (e) 1.0 × 106 EV control and shIfnar1-MOC2-E6/E7 cells were implanted s.c. Upon harvesting, the tumors were homogenized in RIPA buffer and protein extracted from the control and Ifnar1-deficient tumors (n = 3). The tumors were selected randomly. The expression levels of Cd44 were assessed by immunoblotting