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. 2021 May 25;35(12):3466–3481. doi: 10.1038/s41375-021-01282-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Serial examination of 3CAR or 7CAR scFv RNA sequences 48 h and 96 h after electroporation with SpCas9 (SP3/SP7) or coBE3 (BE3/BE7) mRNA and again at end of production on d14. A Amplicons of 3CAR (left) and 7CAR (right) vH and vL sequences with antigen-binding regions (ABR) displayed mapped as a Heatmap in R using the gplots library for C>N conversion rates at the marked sites. B 3CAR (left) and 7CAR (right) scFv ABR mapped as a Heatmap for C > T conversion rates. C Stacked histogram showing <2% C > T changes in each region (ABR1H, ABR2H, ABR3H etc and outside the binding regions – (‘rest’) in SpCas-CAR3, SpCas-CAR7, BE-CAR3, BE-CAR7 or untransduced (UTD) samples.