Table 4.
Cells with large Sup35 foci (%) after Sis1 depletion
| Sis1 Depleted? | 2nd copy of Sis1 | Hours Sis1 depleted |
|
|---|---|---|---|
| 0 | 24 | ||
| Yes | Wild type | 14 ± 2 | 6 ± 3 |
| No | Wild type | 8 ± 3 | 2 ± 1 |
| Yes | None | 14 ± 1 | 75 ± 8 |
| No | None | 5 ± 2 | 7 ± 2 |
| Yes | JGF | 14 ± 3 | 56 ± 7 |
| No | JGF | 12 ± 3 | 20 ± 5 |
Values are average percentages of cells (±SD) with visually obvious bright foci of fluorescent Sup35NGMC from cultures shown in Figure 4. Different pools of 200–420 cells were scored blindly by two people and the data were combined to derive each value. Cells carry pRS314Sis1FRT, the indicated second copy of Sis1, and either pRS315Flp (yes) or pRS315 empty vector (no). Sis1 was depleted by galactose-induced Flp-mediated excision of SIS1 from RS314Sis1FRT. Cells were scored immediately after the shift to galactose (0) and 24 h later.