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. 2021 Nov 17;24(12):103445. doi: 10.1016/j.isci.2021.103445

Figure 2.

Figure 2

Treatment of EAE with heat shock protein gp96

(A and B) Female C57BL/6 mice were immunized with 200 μg gp96 or saline (control) before induction of EAE. Disease score (A) and body weight changes (B) were monitored daily after EAE induction. (C) Spinal cord collected at day 18 from indicated mice were stained with hematoxylin and eosin to assess inflammation. Scale bars, 500 μm.

(D–G) Absolute numbers of CD8+ T cells (D), CD4+ T cells (E), IL-17A-producing CD4+ T cells (F) and IFN-γ-producing CD4+ T cells (G) from indicated organs of mice were evaluated by flow cytometry.

(H) Percentages of Foxp3+ Tregs in the indicated organs of mice. The data are representative of two independent experiments with similar results. n = 5 mice/group. Mean ± SD is shown. The Student's t-test or two-way ANOVA was used for statistical analysis. P < 0.05 was considered statistically significant. ns = not significant.