| cell line (Homo sapiens) |
HeLa CCL2 |
ATCC |
RRID:CVCL_0030
|
|
| cell line (Homo sapiens) |
HeLa Kyoto |
ATCC |
RRID:CVCL_1922
|
|
| cell line (Homo sapiens) |
Neuro-2a |
ATCC |
RRID:CVCL_0470
|
|
| cell line (Homo sapiens) |
HEK293 |
ATCC |
RRID:CVCL_6910
|
|
| cell line (monkey) |
Cos-1 |
ATCC |
RRID:CVCL_023
|
|
| antibody |
anti-GFP (Rabbit polyclonal) |
Abcam |
RRID:AB_305564
|
IEM(1:100) |
| antibody |
anti-LC3b (Rabbit polyclonal) |
Cell Signalling Technology |
RRID:AB_2137707
|
IF(1:400) |
| antibody |
anti-GalT (Rabbit polyclonal) |
Sigma |
RRID:AB_1078254
|
IF(1:50) |
| antibody |
anti-mCherry (Goat polyclonal) |
St John’s laboratory |
STJ140001 |
IEM(1:100) |
| antibody |
anti-myc (Mouse monoclonal, 9E10) |
Sigma |
RRID:AB_2533008
|
WB(1:2000) |
| antibody |
anti-rabbit coupled to 10 nm Gold (Goat, IgG) |
BB International |
RRID:AB_2715527
|
IEM (1:100) |
| antibody |
anti-mouse coupled to 5 nm Gold (Goat, IgG) |
BB International |
RRID:AB_1769168
|
IEM (1:100) |
| antibody |
anti-rabbit coupled to 5 nm Gold (Donkey, IgG) |
Jackson Immuno Research |
RRID:AB_2340610
|
IEM (1:100) |
| antibody |
anti-goat (Mouse, IgG) |
Jackson Immuno Research |
RRID:AB_2339054
|
IEM (1:100) |
| recombinant DNA reagent |
mApple-Rab5a (Plasmid) |
|
RRID:Addgene_54944
|
|
| recombinant DNA reagent |
mApple-Rab7a (Plasmid) |
|
RRID:Addgene_54945
|
|
| recombinant DNA reagent |
GalT-mCherry (Plasmid) |
|
RRID:Addgene_55052
|
|
| recombinant DNA reagent |
mNeptune2-C1 (Plasmid) |
|
RRID:Addgene_54836
|
|
| recombinant DNA reagent |
mApple-Lamp1-pHluorin (Plasmid) |
|
RRID:Addgene_54918
|
|
| recombinant DNA reagent |
GFP-Rab11a (Plasmid) |
|
RRID:Addgene_12674
|
|
| recombinant DNA reagent |
EGFP-2xFYVE (Plasmid) |
|
RRID:Addgene_140047
|
|
| recombinant DNA reagent |
Lamp1-GFP (Plasmid) |
|
RRID:Addgene_34831
|
|
| recombinant DNA reagent |
pSpCas9(BB)–2A-GFP (pX458) (Plasmid) |
|
RRID:Addgene_48138
|
|
| recombinant DNA reagent |
pSpCas9(BB)–2A-puro (pX459) (Plasmid) |
|
RRID:Addgene_48139
|
|
| recombinant DNA reagent |
Snx1-turboGFP (Plasmid) |
Origene |
Origene # RG201844 |
|
| recombinant DNA reagent |
Ccz1-myc (Plasmid) |
Origene |
Origene # RC222195 |
|
| recombinant DNA reagent |
mTagBFP2-Rab5 (Plasmid) |
|
RRID:Addgene_55322
|
|
| recombinant DNA reagent |
mCherry-tagged anti-GFP (VHH) (Plasmid) |
|
RRID:Addgene_109421
|
|
