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. 2021 Dec 3;10(12):84. doi: 10.1038/s41389-021-00374-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A The CCK8 assay was performed to study the effects of CCT8 on CRC cell proliferation in loss- and gain-of-function analysis. B Representative figures and data of colony formation assay in indicated cells. Bars of the right panel represented the number of formed cells. C Bars represent apoptosis in vitro after treatment of fluorouracil for 12 h. D Bars represent the cell cycle analysis in CCT8 overexpressed and silenced CRC cell lines. E Resected xenograft tumors were injected with RKO/LV-CCT8 and RK0/LV-NC. The tumor growth curve over time and the final tumor weight in the scatter plot graph. F Representative figures of HE staining of subcutaneous tumor were shown. Proliferative ability was measured by the Ki-67 proliferative labeling index. Bars of the right panel represent the Ki-67 index of subcutaneous tumor.