The authors regret the presence of undisclosed image splicing in the western blots shown in Figure 4A of their article (1).
Original Figure 4A. The XPB band 4 has been spliced in.
The initial Western blot (XPB image shown below) included 8 samples. The last two samples (lanes 7, 8) were duplicates of 041 UV- and 041 UV+ (lanes 3, 4) in reverse order, for the purpose of Western blotting quality control. The XPB band (loading control) in 041 UV + sample (lane 4) did not blot well. Another XPB band from the duplicate loading of the same sample (lane 7) was used to replace this band.
Original XPB blot – In the published figure, the 041 UV + band (4th from the left) has been replaced by the duplicate lane (7th lane)
The original XPC blot is also shown below for information.
Original XPC blot
A new Figure 4A is provided below.
New Figure 4. A. p53 and DDB2 are involved in UV-induced XPC modification by SUMO-1 and ubiquitin (A) OSU-2, p53-deficient 041 and DDB2-deficient XP-E cells were UV irradiated at 20 J/m2 and incubated for 1 h. The cell lysates were subjected to western blotting with anti-XPC antibody.
This change does not affect the results, discussion and conclusions presented in the article.
Contributor Information
Qi-En Wang, Department of Radiology, The Ohio State University, 103 Wiseman Hall, 400 W. 12th Avenue, Columbus, OH 43210, USA.
Qianzheng Zhu, Department of Radiology, The Ohio State University, 103 Wiseman Hall, 400 W. 12th Avenue, Columbus, OH 43210, USA.
Gulzar Wani, Department of Radiology, The Ohio State University, 103 Wiseman Hall, 400 W. 12th Avenue, Columbus, OH 43210, USA.
Mohamed A El-Mahdy, Department of Radiology, The Ohio State University, 103 Wiseman Hall, 400 W. 12th Avenue, Columbus, OH 43210, USA.
Jinyou Li, Department of Radiology, The Ohio State University, 103 Wiseman Hall, 400 W. 12th Avenue, Columbus, OH 43210, USA.
Altaf A Wani, Department of Radiology, The Ohio State University, 103 Wiseman Hall, 400 W. 12th Avenue, Columbus, OH 43210, USA; Department of Molecular and Cellular Biochemistry, The Ohio State University, 103 Wiseman Hall, 400 W. 12th Avenue, Columbus, OH 43210, USA; James Cancer Hospital and Solove Research Institute, The Ohio State University, 103 Wiseman Hall, 400 W. 12th Avenue, Columbus, OH 43210, USA.
REFERENCES
- 1. Wang Qi-En, Zhu Q., Wani G., El-Mahdy M.A., Li J., Wani A.A.. DNA repair factor XPC is modified by SUMO-1 and ubiquitin following UV irradiation. Nucleic Acids Res. 2005; 33:4023–4034. [DOI] [PMC free article] [PubMed] [Google Scholar]