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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Methods. 2021 Jun 6;200:80–86. doi: 10.1016/j.ymeth.2021.06.005

Figure 2.

Figure 2.

Evaluation of newly developed MMA, ADMA and SDMA antibodies by WB. 293T was treated with different PRMT inhibitors for 48 hours, whole cell lysates were harvested and analyzed with MMA (A), ADMA (B) or SDMA (C) antibodies. For each antibody project, the pre-bleed sera was also tested. All the antibodies were used at 1:2000 dilution. The PRMT inhibitors used include MS023 (Type I PRMT inhibitor), EPZ015666 (PRMT5 inhibitor), and TP064 (CARM1 inhibitor). For each antibody type (MMA, SDMA & ADMA) two rabbits were immunized with the antigen depicted in Figure 1, and the individual rabbits were referred to as TX1 and TX2.