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. 2021 Dec 7;12(6):e02980-21. doi: 10.1128/mBio.02980-21

TABLE 3.

List of qPCR primers

List of RT-qPCR primers
Name of gene Forward primer Reverse primer
ADK GACACAAGCCCTGCCAAAGAT TCAGAGACCAGTTGAGACAGAA
CMSS1 GCCAATGATTTGACTCACAGTCT CTGAATGCTGTCATCGACCTAAT
IRF2BP2 CCCATGACTCCTACATCCTCTT GAGGGCGGACTGTTGCTATTC
NF1 AGATGAAACGATGCTGGTCAAA CCTGTAACCTGGTAGAAATGCGA
CHD9 GAAATGCTAGAAAGGTTGGAGG CGGGACCAGTGAGAATACGTT
FAM19A TGTTAAAACGGGAACTTGTGAGG AAGCATCCACACATGATGGAG
SCN9A ATTCGTGGCTCCTTGTTTTCTG CTACTGGCTTGGCTGATGTTAC
RHOF CCCCATCGGTGTTCGAGAAG GGCCGTGTCGTAGAGGTTC
GRIK5 CCACCGTGAGCCATATCTGTG CGCGAAGCGAAGGTACTGAA
USP15 CGACGCTGCTCAAAACCTC TCCCATCTGGTATTTGTCCCAA
EVL CTTCCGTGATGGTCTACGATG TGCAACTTGACTCCAACGACT
EXOSC8 CGGTTCAATTAGTACCGCAGAT ACGTATCCTTTATCAGGGGCAT
COPS5 TGGGTCTGATGCTAGGAAAGG CTATGATACCACCCGATTGCATT
SLC7A6 CGGGCTTTCAGTTGTTAGACC ACACAGTCAATCGCTCACGG
EVI2B ACCAACACAATTCAGCGACAC GTTGTAGGCAAGTGGTTGTCC
SPN GCTGGTGGTAAGCCCAGAC GGCTCGCTAGTAGAGACCAAA
POL GGTTTATTACAGGGACAGCAGAGA ACCTGCCATCTGTTTTCCATA
GAG AGTAGTGTGTGCCCGTCTGT TCGCTTTCAGGTCCCTGTTCG
TAT CAAAAGCCTTAGGCATCTCCT CCACCTTCTTCTTCGATTCCT
β2Microglobulin AGCGTACTCCAAAGATTCAGGTT ATGATGCTGCTTACATGTCTCGAT
GAPDH CAAGAAGGTGGTGAAGCAG GCCAAATTCGTTGTCATACC
List of ChIP-qPCR primers
Amplicon Forward primer Reverse primer
HIV-1Nuc0 (5′ + 3′ LTR) CCACACACAAGGCTACTTCC AACTGGTACTAGCTTGTAGCAC
HIV-1 HSS1 (5′ + 3′ LTR) TGTGAGCCTGCATGGGATGG GAAAGTCCCCAGCGGAAAGT
HIV-1Nuc1/HSS2 (5′ + 3′ LTR) CGTCTGTTGTGTGACTCTGGT TCGAGAGAGCTCCTCTGGTT
HIV-1HSS2/Nuc2 (5′ LTR) GCCCGAACAGGGACTTGAAA TTGGCGTACTCACCAGTCG
HIV-1Nuc2/Gag (5′ LTR) GGTGCGAGAGCGTCAGTAT AGCTCCCTGCTTGCCCATA
Hk2 GCCGACTCTTGTATTGCCTG TATTGTAGCACGGCCGGAAA
GFP (HIV-1) GGAGTGGTCCCAGTTCTTGTTG ACAGGTAGCTTCCCAGTAGTGC