TABLE 3.
List of RT-qPCR primers | ||
---|---|---|
Name of gene | Forward primer | Reverse primer |
ADK | GACACAAGCCCTGCCAAAGAT | TCAGAGACCAGTTGAGACAGAA |
CMSS1 | GCCAATGATTTGACTCACAGTCT | CTGAATGCTGTCATCGACCTAAT |
IRF2BP2 | CCCATGACTCCTACATCCTCTT | GAGGGCGGACTGTTGCTATTC |
NF1 | AGATGAAACGATGCTGGTCAAA | CCTGTAACCTGGTAGAAATGCGA |
CHD9 | GAAATGCTAGAAAGGTTGGAGG | CGGGACCAGTGAGAATACGTT |
FAM19A | TGTTAAAACGGGAACTTGTGAGG | AAGCATCCACACATGATGGAG |
SCN9A | ATTCGTGGCTCCTTGTTTTCTG | CTACTGGCTTGGCTGATGTTAC |
RHOF | CCCCATCGGTGTTCGAGAAG | GGCCGTGTCGTAGAGGTTC |
GRIK5 | CCACCGTGAGCCATATCTGTG | CGCGAAGCGAAGGTACTGAA |
USP15 | CGACGCTGCTCAAAACCTC | TCCCATCTGGTATTTGTCCCAA |
EVL | CTTCCGTGATGGTCTACGATG | TGCAACTTGACTCCAACGACT |
EXOSC8 | CGGTTCAATTAGTACCGCAGAT | ACGTATCCTTTATCAGGGGCAT |
COPS5 | TGGGTCTGATGCTAGGAAAGG | CTATGATACCACCCGATTGCATT |
SLC7A6 | CGGGCTTTCAGTTGTTAGACC | ACACAGTCAATCGCTCACGG |
EVI2B | ACCAACACAATTCAGCGACAC | GTTGTAGGCAAGTGGTTGTCC |
SPN | GCTGGTGGTAAGCCCAGAC | GGCTCGCTAGTAGAGACCAAA |
POL | GGTTTATTACAGGGACAGCAGAGA | ACCTGCCATCTGTTTTCCATA |
GAG | AGTAGTGTGTGCCCGTCTGT | TCGCTTTCAGGTCCCTGTTCG |
TAT | CAAAAGCCTTAGGCATCTCCT | CCACCTTCTTCTTCGATTCCT |
β2Microglobulin | AGCGTACTCCAAAGATTCAGGTT | ATGATGCTGCTTACATGTCTCGAT |
GAPDH | CAAGAAGGTGGTGAAGCAG | GCCAAATTCGTTGTCATACC |
List of ChIP-qPCR primers | ||
Amplicon | Forward primer | Reverse primer |
HIV-1Nuc0 (5′ + 3′ LTR) | CCACACACAAGGCTACTTCC | AACTGGTACTAGCTTGTAGCAC |
HIV-1 HSS1 (5′ + 3′ LTR) | TGTGAGCCTGCATGGGATGG | GAAAGTCCCCAGCGGAAAGT |
HIV-1Nuc1/HSS2 (5′ + 3′ LTR) | CGTCTGTTGTGTGACTCTGGT | TCGAGAGAGCTCCTCTGGTT |
HIV-1HSS2/Nuc2 (5′ LTR) | GCCCGAACAGGGACTTGAAA | TTGGCGTACTCACCAGTCG |
HIV-1Nuc2/Gag (5′ LTR) | GGTGCGAGAGCGTCAGTAT | AGCTCCCTGCTTGCCCATA |
Hk2 | GCCGACTCTTGTATTGCCTG | TATTGTAGCACGGCCGGAAA |
GFP (HIV-1) | GGAGTGGTCCCAGTTCTTGTTG | ACAGGTAGCTTCCCAGTAGTGC |