Synthesis of a Coumarin-Based Analogue of Schweinfurthin F

Abstract

The natural schweinfurthins are stilbenes with significant antiproliferative activity and an uncertain mechanism of action. To obtain a fluorescent analogue with minimal deviation from the natural structure, a coumarin ring system was annulated to the D-ring, creating a new analogue of schweinfurthin F. This stilbene was prepared through a convergent synthesis, with a Horner–Wadsworth–Emmons condensation employed to form the central stilbene olefin. After preparation of a tricyclic phosphonate via a recent and more efficient modification of the classic Arbuzov reaction, condensation was attempted with an appropriately substituted bicyclic aldehyde but the coumarin system did not survive the reaction conditions. When olefin formation preceded generation of the coumarin, the stilbene formation proceeded smoothly and ultimately allowed access to the targeted coumarin-based schweinfurthin analogue. This analogue displayed the desired fluorescence properties along with significant biological activity in the National Cancer Institute’s 60-cell line bioassay, and the pattern of this biological activity mirrored that of the natural product schweinfurthin F. This approach gives facile access to new fluorescent analogues of the natural schweinfurthins and should be applicable to other natural stilbenes as well.

Introduction

The schweinfurthins (Figure 1) are a small group of natural products isolated, at least thus far, from plants of the genus Macaranga (Euphorbiaceae) directly^1,2 or indirectly from propolis produced by bees visiting Macaranga plants.³ The combination of an unusual pattern of differential activity in the National Cancer Institute’s (NCI’s) 60-cell line screen^4,5 and isolation efforts that have resulted in limited and sometimes poorly reproducible quantities⁶ has encouraged us to pursue efforts to synthesize these compounds and a variety of analogues. To date, we have reported the total synthesis of several natural schweinfurthins that include the hexahydroxanthene system [A (1),⁷ B (2),⁸ E (3),⁸ F (5),⁹ G (6),¹⁰ and vedelianin (4)],¹¹ including one as both enantiomers to allow determination of the absolute stereochemistry of the natural products (Figure 1). We also have prepared approximately 90 analogues that have been evaluated in the NCI’s 60-cell line screen for structure activity studies.^7−9,12,13 Results of these studies indicate that the A/B/C ring system and a stilbene in the trans orientation are essential to the selective antiproliferative activity of these compounds, while modifications of the D-ring are generally better tolerated. Of special significance, studies of structure–activity relationships and chemical stability have revealed that the D-ring resorcinol may limit the schweinfurthins’ stability. Thus, optimum placement of a coumarin system might preserve the biological activity and simultaneously improve the chemical stability. Methylation of one of the symmetric D-ring phenolic groups has been shown to significantly increase chemical stability and testing in the NCI-60 assay revealed that there is little or no loss in activity relative to the corresponding non-methylated compounds. Furthermore, the indole analogues 7 and 8 also have shown significant activity, suggesting a tolerance for substitution at a single phenolic position.¹⁴

Figure 1.

Relevant natural schweinfurthins and two indole analogues.

The NCI created the COMPARE algorithm to associate bioactivity data from each candidate in the NCI-60 bioassay with those from other compounds that have also been through the screening process and function by a similar mechanism of action rather than by structural similarity.¹⁵ In the COMPARE analysis, the schweinfurthin family did not pose biological resemblance to that of any chemotherapeutic agent currently in use, but rather, the activity of the schweinfurthin family most closely resembles that of the cephalostatins (e.g. 9), the ritterazines (e.g. 10), the stellettins (e.g. 11), and OSW-1 (12, Figure 2).¹³ Deeper investigation into the mechanism of action of the schweinfurthins by several groups has not yet provided a complete and clear mode of action. Studies have suggested interactions between several targets including oxysterol binding proteins,¹⁶⁻¹⁸ trans-Golgi-network trafficking,¹⁹ and the production and export of cholesterol²⁰ and other products of isoprenoid biosynthesis.²¹

Figure 2.

Natural products that display biological activity similar to that of schweinfurthins, based on COMPARE analyses.

To increase understanding of the mechanism of action for the schweinfurthins, it might be useful to prepare a fluorescent analogue, as long as that analogue displays significant biological activity. To maximize the possibility of activity, it appeared prudent to anneal a coumarin ring to the D-ring, as suggested in structure 13 (Figure 3). Compound 13 would preserve the complete hexahydroxanthene system of a mono-methylated schweinfurthin F (14), the trans-stilbene, and one free phenol in the D-ring. Thus, preparation of the schweinfurthin analogues in the form of structure 13 became our goal.

Figure 3.

Comparison of a coumarin-containing schweinfurthin (13) with a mono-methylated schweinfurthin F (14).

Results and Discussion

Although several disconnections have been explored for schweinfurthin assembly,^1,22,23 we have favored use of late-stage Horner–Wadsworth–Emmons (HWE) condensation to form the central stilbene olefin because this approach allows a highly convergent synthesis. From this perspective, the coumarin-containing schweinfurthin F analogue 13 could be seen arising from an HWE olefination between phosphonate 15 and aldehyde 16 (Scheme 1). Phosphonate 15 may be formed from the known tricyclic alcohol 17,¹⁰ which can be prepared in high enantiomeric excess from commercial vanillin (18) through an enantioselective Shi epoxidation.²⁴ If the HWE condensation were postponed to the end of the synthetic sequence, the complementary aldehyde 16 would be required. Coumarin 16 could be prepared via a Knoevenagel condensation between the aldehyde 19 and a β-ketoester of the general structure 20, with ethyl acetoacetate providing the methyl ketone and extended acetoacetates giving larger analogues. Aldehyde 19 could be seen to arise from bromide 21 by halogen metal exchange followed by reaction with dimethylformamide (DMF). Finally, the benzyl methyl ether 21 could be obtained from commercial 3,5-dihydroxybenzoic acid (22).

Scheme 1. One Retrosynthesis to the Schweinfurthin Analogue 13.

Initial synthetic efforts were focused on the coumarin component because previous syntheses of other schweinfurthins provided confidence that an appropriate tricyclic component could be prepared. When compounds 13 and 16 include a methyl ketone, this group can be viewed as a mimic of the prenyl group in schweinfurthin F and a homoprenyl ketone could be imagined to mimic the geranyl substituent of larger schweinfurthins. Our efforts began with the prenyl mimic where R is a methyl group because this allowed use of commercially available ethyl acetoacetate as the ketoester 20.

Although the brominated resorcinol 23 is commercially available, it can be easily prepared in virtually quantitative yield by treatment of 3,5-dihydroxybenzoic acid (22) with bromine (Scheme 2). The benzoic acid 23 has been converted to the benzylic alcohol 25 through a three-step sequence via the methyl ester,^25,26 but it also was possible to accomplish this overall transformation in just two steps by formation of the acyloxyester 24 while concurrently protecting the phenols as MOM ethers, followed by reduction of this intermediate to the desired alcohol 25. Protection of the benzylic alcohol 25 as the methyl ether 21 proceeded smoothly,²⁷ and halogen metal exchange followed by treatment with DMF afforded the desired aldehyde 19.

Scheme 2. Synthesis of the Aldehyde Intermediate 19.

With the aldehyde 19 in hand, attention was turned to formation of the desired coumarin ring system through a Knoevenagel condensation. All efforts to form a coumarin directly from the bis-MOM-protected compound 19 went unrewarded. Fortunately, treatment of compound 19 with sodium bisulfate on silica resulted in cleavage of a single MOM protecting group in reasonably good yield (70%, Scheme 3).^28,29 Grinding the resulting ortho-hydroxy benzaldehyde 26 with ethyl acetoacetate (27) and piperidine resulted in condensation and cyclization to afford the coumarin 28. Because ketones can undergo reaction with DDQ via their tautomeric enol forms, the ketone 28 was protected as its acetal 29. Subsequent reaction with DDQ gave the desired coumarin aldehyde 30 in modest yield. Preparation of the tricyclic phosphonate 15 then was investigated because aldehyde 30 appeared to be an appropriate substrate for an HWE condensation.

Scheme 3. Formation of the Coumarin Aldehyde 30.

The tricyclic phosphonate 15 was employed in our original synthesis of schweinfurthin F,¹⁰ where it was prepared from the corresponding alcohol 17 in an overall yield of 62% via a classical approach involving formation of the mesylate, displacement by sodium iodide, and an Arbuzov reaction with triethyl phosphite. Instead, a shortened Arbuzov approach was followed. After the aldehyde 31 was prepared via DDQ oxidation of the corresponding benzyl methyl ether, the C-2 alcohol was protected as the MOM ether (32) and the aldehyde was reduced to the benzylic alcohol 17 (Scheme 4). Then the alcohol 17 simply was treated with zinc iodide and triethyl phosphite, modernized Arbuzov conditions³⁰ for benzylic alcohols, which gave phosphonate 15 in a single step and 69% isolated yield.

Scheme 4. Direct Conversion of Benzylic Alcohol 17 to Phosphonate 15.

From all perspectives, the HWE condensation of phosphonate 15 and aldehyde 30 was expected to be straightforward, and parallel reactions have been employed in multiple schweinfurthin syntheses. To our disappointment, this specific HWE condensation failed despite repeated attempts (Scheme 5) perhaps because the coumarin subunit was not stable under the reaction conditions. Whatever the root cause, the failure of this reaction necessitated a redesign of the synthetic sequence.

Scheme 5. Initially Attempted HWE Condensation.

To take full advantage of the intermediates in hand and the experience gained with the successful reactions described above, introduction of the coumarin ring system was postponed until after the formation of the central stilbene. Therefore, the alcohol 25 was protected as its TBS ether 34 to avoid potential side-reactions during DDQ oxidation (Scheme 6). Compound 34 undergoes lithium halogen exchange under standard conditions, and a subsequent reaction with DMF gave the aldehyde 35. After protection of the carbonyl group as its acetal 36, treatment with TBAF generated the primary alcohol 37. Final MnO₂ oxidation provided the new HWE coupling partner, aldehyde 38.

Scheme 6. Synthesis of the Aldehyde 38.

The HWE condensation of phosphonate 15 and aldehyde 38 proceeded smoothly upon treatment with sodium hexamethyldisilazide, affording the schweinfurthin analogue 39 in quantitative yield based on recovered phosphonate 15 (Scheme 7). The three MOM acetals and the ethylene glycol-protected aldehyde underwent hydrolysis under acidic conditions to afford the aldehyde 40. After aldehyde 40 was combined with ethyl acetoacetate (27) and piperidine and the reaction was allowed to stir in anhydrous MeOH, the desired fluorescent coumarin schweinfurthin analogue 41 was obtained in high enantiomeric excess.¹⁰ The spectral data for coumarin 41 indicates that the compound has an absorption maximum at ∼420 nm and an emission maximum at ∼590 nm, showing the expected fluorescence.

Scheme 7. Assembly of the First Coumarin-Based Schweinfurthin (41).

In principal, synthesis of other coumarin-based schweinfurthin analogues could be based on C-alkylation of the methyl ketone in compound 41. However this would certainly require protection of the free phenol and probably the C-2 hydroxyl group as well. A more attractive approach might involve extension of ethyl acetoacetate (27) prior to the Knoevenagel condensation. To test this possibility, the β-ketoester 42 was prepared via alkylation of the ethyl acetoacetate dianion (Scheme 8).³¹ When compound 42 was ground in a mortar with a pestle in the presence of piperidine to induce condensation and cyclization by mechanochemical means, the extended coumarin 43 was obtained in an attractive yield. Although synthesis of compound 43 demonstrates the accessibility of more extended coumarins, pursuit of additional schweinfurthin analogues was postponed pending the results of bioassays on the new analogues in hand.

Scheme 8. Synthesis of Coumarin 43 with an Extended Isoprenoid Chain.

Both schweinfurthin analogues 40 and 41 were tested in the NCI-60 cell line bioassay. Both compounds were first tested in a single-dose assay and demonstrated sufficient activity to warrant testing in the full five-dose assay. The aldehyde 40 shows modest activity against SF-295 with a GI₅₀ of 3.0 μM (Table 1 and Supporting Information). This activity is 270 times less potent than that of natural schweinfurthin A (1). Although aldehyde 40 is not as active as most schweinfurthins and schweinfurthin analogues sent to the NCI, it does show a pattern of activity similar to that of other schweinfurthins, with a Pearson correlation coefficient of 0.66 to schweinfurthin A.³² The GI₅₀ against each cell line in the NCI-60 assay for compound 40 shows a pattern similar to that of other schweinfurthins. Of the cell lines tested, analogue 40 also has the greatest activity against the CNS cancer cell line SF-539 with a GI₅₀ of 1.3 μM, which aligns with our expectation that the schweinfurthins have selective activity against CNS malignancies.

Table 1. Comparison of the Activity (GI₅₀) of Compounds 40 and 41 to Representative Schweinfurthins against Selected CNS Cell Lines.

compound # (NSC number)	SF-295 (μM)	SF-539 (μM)	SNB-75 (μM)	Pearson correlation to 1
1 (696119)	0.011	0.010	0.015	1.00
44 (730430)a	1.5	–	15.8	0.39
14 (740545)	0.066	0.28	0.18	0.78
40 (819974)	3.0	1.3	1.8	0.66
41 (823234)	0.51	0.98	1.0	0.66

^aFigure 4.

The five-dose assay of coumarin 41 also shows selective activity toward some cancer cell lines over others (c.f. Supporting Information) in a pattern of activity strikingly similar to that of other schweinfurthins that carry a substituent para to the stilbene linkage (i.e. more substituted variations on the parent compound 44, Figure 4). Among the most sensitive cell lines were the SF-295 and SF-539 human-derived glioblastoma lines, with GI₅₀ values of 0.51 and 0.98 μM respectively, but cells in the leukemia (RPMI-8226, 0.32 μM) and renal (RXF 393, 0.43 μM) panels also were sensitive as is often the case with other schweinfurthins. Conversely, the ovarian cancer panel was uniformly resistant, which is also typical of the schweinfurthins. The three-fold increase in potency of the analogue 41 relative to its precursor, aldehyde 40, also is striking and encouraging.

Figure 4.

Parent compound 44.

In conclusion, two new schweinfurthin analogues, coumarin 41 and its immediate precursor aldehyde 40, have been synthesized and tested for biological activity in the NCI-60 cell bioassay. Although the initial approach to the central stilbene demonstrated only that this coumarin system did not survive the standard HWE reaction conditions, it proved possible to incorporate the coumarin ring system after formation of the central stilbene. Both traditional Knoevenagel condensation and mechanical grinding of an ortho hydroxy aldehyde with a β-ketoester allowed formation of the coumarin system. Furthermore, the target compound 41 displays both significant antiproliferative activity in the NCI 60-cell line screen and fluorescent properties that may help illuminate the mechanism of action for the schweinfurthins. Finally, the reactions and strategies reported here might be applicable to preparation of fluorescent analogues of other natural stilbenes, including compounds such as combretastatin,³³ resveratrol^34,35 and its myriad derivatives,³⁶ the chiricanines,³⁷ the arachidins and arahypins,³⁸ and the pawhuskins.³⁹

Experimental Section

General Section

Diethyl ether (Et₂O) and tetrahydrofuran (THF) were distilled from sodium and benzophenone, and dichloromethane (CH₂Cl₂) was distilled from calcium hydride prior to use. Solutions of n-BuLi were purchased from commercial sources and titrated with diphenylacetic acid to determine molar concentrations prior to use. All other reagents and solvents were purchased from commercial sources and used without further purification. The nuclear magnetic resonance (NMR) spectra were obtained on 300, 400, or 600 MHz Bruker spectrometers with Si(CH₃)₄ (¹H, δ 0.00), CDCl₃ (¹H, δ 7.26; ¹³C, δ 77.2), CD₃CN (¹H, δ 1.94; ¹³C, δ 118.3, 1.32), or (CD₃)₂CO (¹H, δ 2.05; ¹³C, δ 206.3, 29.8) as internal standards. To assign signals as C, CH, CH₂, or CH₃, DEPT-135 NMR spectra were obtained. High-resolution mass spectra were obtained at the University of Iowa Mass Spectrometry Facility. Silica gel (60 Å, 0.040–0.063 mm) was used for flash column chromatography. The UV–vis spectra were obtained on a Cary UV–vis NIR spectrophotometer, and fluorescence data were collected on HORIBA Scientific FluroMax-4. A quartz (200–2500 nm) 1400 μL Hellma Analytics cuvette (semi-micro cell type 114F-QS) with a 10 mm × 4 mm path length fitted with a PTFE stopper was used for UV–vis and fluorometry.

4-Bromo-3,5-dihydroxybenzoic Acid (23)

To an oven-dried and argon-purged round-bottom flask containing 3,5-dihydroxybenzoic acid (22, 10.0 g, 64.5 mmol) was added aqueous 20% HCl (110 mL) followed by a dropwise addition of bromine (3.31 mL, 64.5 mmol). The reaction was heated in an oil bath under reflux for 3 h and then was quenched by addition of ice. The solution was washed with Et₂O (3 × 50 mL), and the combined organic layers were dried (Na₂SO₄) and then filtered through a bed of Celite, and the filtrate was concentrated on a rotary evaporator to afford aryl bromide 23 as an off-white solid (14.9 g, 99%). Both the ¹H NMR and ¹³C NMR spectra were in agreement with the reported data.⁴⁰

Methoxymethyl 4-Bromo-3,5-bis(methoxymethoxy)benzoate (24)

To an oven-dried and argon-purged round-bottom flask containing the carboxylic acid 23 (400 mg, 1.7 mmol) in CH₂Cl₂ (20 mL) at 0 °C was added dropwise DIPEA (1.0 mL, 6.0 mmol) followed by a dropwise addition of MOMCl (520 μL, 6.9 mmol). The reaction was allowed to stir at 0 °C for 2.5 h, and then the reaction was quenched by addition of saturated NH₄Cl (10 mL) and the organic compounds were extracted into CH₂Cl₂ (3 × 20 mL). The combined organic layers were washed with 3 N NaOH (15 mL) and dried (Na₂SO₄), and the solids were removed by filtration. The filtrate was concentrated on a rotary evaporator to afford compound 24 as a white solid (0.58 g, 93%): ¹H NMR (400 MHz, CDCl₃): δ 7.51 (s, 2H), 5.46 (s, 2H), 5.30 (s, 4H), 3.53 (s, 3H), 3.52 (s, 6H); ¹³C{¹H} NMR (101 MHz, CDCl₃): δ 165.3, 154.9 (2C), 130.0, 110.2 (2C), 109.9, 95.1 (2C), 91.2, 57.9, 56.6 (2C). HRMS (ESI) m/z: [M + Na]⁺ calcd for C₁₃H₁₇O₇BrNa, 387.0055; found, 387.0060.

[4-Bromo-3,5-bis(methoxymethoxy)phenyl]methanol (25)

To an oven-dried and argon-purged round-bottom flask containing compound 24 (4.56 g, 12.5 mmol) in THF (60 mL) was slowly added solid NaBH₄ (4.02 g, 106 mmol), and the reaction was allowed to stir at 65 °C for 15 min. After MeOH (60 mL) was added dropwise, the reaction was heated under reflux in an oil bath for an additional 2 h. After cooling to rt, the reaction was quenched by dropwise addition of saturated NH₄Cl (50 mL). The organic compounds were extracted into EtOAc, the combined organic layers were dried over Na₂SO₄, and the solids were removed by filtration. The filtrate was concentrated on a rotary evaporator to afford the benzylic alcohol 25 as a white solid (2.76 g, 72%): ¹H NMR (400 MHz, CDCl₃): δ 6.85 (s, 2H), 5.26 (s, 4H), 4.64 (s, 2H), 3.52 (s, 6H); ¹³C{¹H} NMR (100 MHz, (CD₃)₂CO): δ 154.8 (2C), 143.6, 107.3 (2C), 101.15, 95.4 (2C), 63.3, 55.6 (2C).²⁵

2-Bromo-1,3-bis(methoxymethoxy)-5-(methoxymethyl)benzene (21)

To a solution of the alcohol 25 (8.66 g, 28.2 mmol) in THF (200 mL) was slowly added 60% NaH in oil (1.27 g, 53.0 mmol), and the reaction then was allowed to stir at 0 °C for 5 min. To the solution was added iodomethane (2.19 mL, 35.3 mmol), and the solution was allowed to stir for 2 h. After the reaction was quenched by addition of H₂O, it was extracted with EtOAc. The combined organic layers were washed with 1 N NaOH, dried over Na₂SO₄, and filtered, and the filtrate was concentrated on a rotary evaporator to afford the methyl ether 21 as colorless crystals (8.71 g, 96%): ¹H NMR (400 MHz, (CD₃)₂CO): δ 6.88 (s, 2H), 5.30 (s, 4H), 4.40 (s, 2H), 3.49 (s, 6H), 3.36 (s, 3H); ¹³C{¹H} NMR (100 MHz, (CD₃)₂CO): δ 154.9 (2 C), 152.0 (C), 139.8 (C), 108.0 (2 CH), 94.9 (2 CH₂), 73.5 (CH₂), 57.4 (CH₃), 55.6 (2 CH₃).²⁷

2,6-Bis(methoxymethoxy)-4-(methoxymethyl)benzaldehyde (19)

To a flame-dried and argon-purged round-bottom flask containing n-BuLi (2.48 M, 5.5 mL, 13 mmol) at −78 °C was added a −78 °C solution of the aryl bromide 21 (3.55 g, 11.1 mmol) in Et₂O (200 mL) using a cannula. The solution was allowed to stir for 15 min and then DMF (0.94 mL, 12 mmol) was added dropwise. Once the reaction reached rt, it was quenched by addition of NH₄Cl and extracted with Et₂O. The combined organic layers were dried over Na₂SO₄ and filtered, and the filtrate was concentrated on a rotary evaporator to afford a yellow oil. Final purification was achieved using an ISCO auto-column (0%–100% EtOAc in hexanes) to afford aldehyde 19 as a yellow oil (1.52 g, 50%): ¹H NMR (400 MHz, CDCl₃): δ 10.51 (s, 1H), 6.82 (s, 2H), 5.27 (s, 4H), 4.42 (s, 2H), 3.50 (s, 6H), 3.41 (s, 3H); ¹³C{¹H} NMR (100 MHz, CDCl₃): δ 188.5 (CH), 159.4 (2 CH), 147.1 (C), 114.9 (C), 106.6 (2 C), 94.6 (2 CH₂), 73.8 (CH₂), 58.3 (CH₃), 56.3 (2 CH₃). HRMS (ESI) m/z: [M + Na]⁺ calcd for C₁₃H₁₈O₆Na, 293.0996; found, 293.0995.

2-Hydroxy-6-(methoxymethoxy)-4-(methoxymethyl)benzaldehyde (26)

A mixture of compound 19 (1.38 g, 5.09 mmol) and NaHSO₄ on SiO₂ (3.06 g)^28,29 was allowed to stir in CH₂Cl₂ (75 mL) at rt for 10 min. The mixture was filtered through Celite, washed with CH₂Cl₂, and the filtrate was concentrated on a rotary evaporator to afford the phenol 26 (806 mg, 70%): ¹H NMR (300 MHz, CDCl₃): δ 11.85 (s, 1H), 10.23 (s, 1H), 6.51 (s, 1H), 6.44 (s, 1H), 5.23 (s, 2H), 4.31 (s, 2H), 3.43 (s, 3H), 3.32 (s, 3H); ¹³C{¹H} NMR (75 MHz, CDCl₃): δ 193.7 (CH), 163.3 (C), 160.0 (C), 150.3 (C), 110.3 (C), 108.4 (CH), 102.2 (CH), 94.5 (CH₂), 73.7 (CH₂), 58.3 (CH₃), 56.4 (CH₃). HRMS (ESI) m/z: [M + H]⁺ calcd for C₁₁H₁₅O₅, 227.0920; found, 227.0913.

3-Acetyl-5-(methoxymethoxy)-7-(methoxymethyl)chromen-2-one (28)

The aldehyde 26 (515 mg, 2.28 mmol) was combined with ethyl acetoacetate (27, 580 μL, 4.55 mmol) and piperidine (nine drops) in a mortar, and the solution was ground with a pestle for 30 min. The residue was transferred to a round-bottom flask with EtOAc, and the solvent was removed using a rotary evaporator. Final purification by crystallization from hot EtOH and H₂O gave the coumarin 28 (329 mg, 50%): ¹H NMR (300 MHz, CDCl₃): δ 8.87 (s, 1H), 6.96 (s, 1H), 6.93 (s, 1H), 5.33 (s, 2H), 4.50 (s, 2H), 3.51 (s, 3H), 3.44 (s, 3H), 2.70 (s, 3H); ¹³C{¹H} NMR (75 MHz, CDCl₃): δ 195.3 (C), 159.6 (C), 157.2 (C), 156.5 (C), 149.1 (C), 142.2 (CH), 123.6 (C), 109.9 (C), 108.1 (CH), 108.0 (CH), 95.1 (CH₂), 74.3 (CH₂), 59.0 (CH₃), 57.0 (CH₃), 30.4 (CH₃). HRMS (ESI) m/z: [M + H]⁺ calcd for C₁₅H₁₇O₆, 293.1020; found, 293.1019.

5-(Methoxymethoxy)-7-(methoxymethyl)-3-(2-methyl-1,3-dioxolan-2-yl)chromen-2-one (29)

To a round-bottom flask containing the ketone 28 (250 mg, 850 μmol) in benzene (20 mL) were added ethylene glycol (0.34 mL, 6.0 mmol) and pyridinium p-toluenesulfonate (43 mg, 0.17 mmol). The solution was heated in an oil bath under reflux with a Dean–Stark trap in place for 18 h. After it had cooled to rt, the reaction was quenched by addition of saturated NaHCO₃ (5 mL) and extracted with EtOAc, and the combined organic layers were dried (Na₂SO₄) and filtered. The filtrate was concentrated by rotary evaporation. Final purification was achieved through flash column chromatography (20% EtOAc in hexanes) to afford the acetal 29 as colorless crystals (110 mg, 39%) and recovered ketone 28 (44 mg, 18%). For the acetal: ¹H NMR (400 MHz, CDCl₃): δ 8.27 (s, 1H), 6.96 (s, 1H), 6.93 (s, 1H), 5.33 (s, 2H), 4.40 (s, 2H), 4.10 (m, 2H), 3.92 (m, 2H), 3.55 (s, 3H), 3.44 (s, 3H), 1.83 (s, 3H); ¹³C{¹H} NMR (100 MHz, CDCl₃): δ 159.4 (C), 154.9 (C), 154.2 (C), 143.8 (C), 133.6 (CH), 126.7 (C), 109.0 (C), 108.1 (CH), 107.2 (CH), 107.1 (C), 94.9 (CH₂), 74.0 (CH₂), 65.0 (2 CH₂), 58.5 (CH₃), 56.6 (CH₃), 24.4 (CH₃). HRMS (ESI) m/z: [M + H]⁺ calcd for C₁₇H₂₁O₇, 337.1282; found, 337.1278.

5-(Methoxymethoxy)-7-methyl-3-(2-methyl-1,3-dioxolan-2-yl)chromen-2-one (30)

To a solution of the methyl ether 29 (110 mg, 330 μmol), CH₂Cl₂ (5 mL), and H₂O (0.5 mL) was added DDQ (210 mg, 900 μmol), and the mixture was allowed to stir at rt for 24 h. The mixture was quenched by addition of saturated NaHCO₃, extracted with CH₂Cl₂, and the combined organic layers were washed with brine, dried (Na₂SO₄), and filtered. The filtrate was concentrated on a rotary evaporator. Final purification was achieved through flash column chromatography (20% EtOAc in hexanes) to afford the aldehyde 30 as a yellow oil (45 mg, 43%): ¹H NMR (400 MHz, CDCl₃): δ 10.00 (s, 1H), 8.29 (s, 1H), 7.48 (s, 1H), 7.43 (s, 1H), 5.38 (s, 2H), 4.12 (m, 2H), 3.95 (m, 2H), 3.53 (s, 3H), 1.82 (s, 3H); ¹³C{¹H} NMR (100 MHz, CDCl₃): δ 190.7 (CH), 158.5 (C), 154.8 (C), 154.7 (C), 138.8 (C), 132.7 (CH), 129.8 (C), 118.1 (C), 111.9 (CH), 107.4 (CH), 107.0 (C), 95.1 (CH₂), 65.1 (2 CH₂), 56.8 (CH₃), 24.5 (CH₃). HRMS (ESI) m/z: [M + H]⁺ calcd for C₁₆H₁₆O₇Na, 343.0788; found, 343.0796.

(7R,8aR,10aR)-4-Methoxy-7-(methoxymethoxy)-8,8,10a-trimethyl-6,7,8a,9-tetrahydro-5H-xanthene-2-carbaldehyde (32)

To a vial containing aldehyde 31(8) (177 mg, 580 μmol) in CH₂Cl₂ (5 mL) at 0 °C was added dropwise DIPEA (110 μL, 0.64 mmol). The solution was allowed to stir for 10 min, and then MOMCl (50 μL, 0.64 mmol) was added dropwise. After the solution was allowed to stir for 72 h, it was quenched by addition of NH₄Cl and was extracted with CH₂Cl₂. The combined organic layers were washed with 1 N NaOH, dried (Na₂SO₄), and filtered, and the filtrate was concentrated in vacuo to afford aldehyde 32 (140 mg, 70%): ¹H NMR (400 MHz, CDCl₃): δ 9.80 (s, 1H), 7.26–7.23 (m, 2H), 4.79 (d, J = 6.9 Hz, 1H), 4.66 (d, J = 7.0 Hz, 1H), 3.90 (s, 3H), 3.41 (s, 3H), 3.31–3.24 (m, 1H), 2.80–2.78 (m, 2H), 2.18 (td, J = 12.9, 3.6 Hz, 1H), 2.02 (dq, J = 14.4, 4.0 Hz, 1H), 1.82 (ddd, J = 13.8, 13.8, 4.1 Hz, 1H), 1.72 (dd, J = 11.3, 7.5 Hz, 1H), 1.65–1.58 (m, 1H), 1.28 (s, 3H), 1.10 (s, 3H), 0.92 (s, 3H); ¹³C NMR (101 MHz, CDCl₃): δ 191.2, 149.6, 148.9, 129.0, 127.4, 122.6, 107.4, 96.3, 83.8, 78.5, 56.1, 55.7, 46.7, 38.4, 37.5, 27.5, 25.4, 23.1, 20.1, 15.2. HRMS (ESI) m/z: [M + H]⁺ calcd for C₂₀H₂₉O₅, 349.2015; found, 349.2006.

[(7R,8aR,10aR)-4-Methoxy-7-(methoxymethoxy)-8,8,10a-trimethyl-6,7,8a,9-tetrahydro-5H-xanthen-2-yl]methanol (17)

To an oven-dried and argon-purged round-bottom flask containing the aldehyde 32 (350 mg, 1.00 mmol) in THF (10 mL) and MeOH (2 mL) at 0 °C was added solid NaBH₄ (234 mg, 1.60 mmol). The solution was allowed to stir for 40 min, was quenched by addition of H₂O, and then was extracted with EtOAc. The combined organic layers were washed with saturated NaHCO₃ and brine and dried (MgSO₄). After filtration, the filtrate was concentrated on a rotary evaporator to afford the benzylic alcohol 17 (352 mg, 100%) as a colorless oil. Both the ¹H and ¹³C NMR spectra were in agreement with data reported in the literature.¹⁰

[(2R,4aR,9aR)-7-(Diethoxyphosphonylmethyl)-5-methoxy-1,1,4a-trimethyl-3,4,9,9a-tetrahydro-2H-xanthen-2-yl]oxymethanol (15)

To an oven-dried and argon-purged round-bottom flask containing ZnI₂ (1.1 g, 3.5 mmol) and triethyl phosphite (400 μL, 2.3 mmol) in THF (15 mL) was added benzylic alcohol 17 (410 mg, 1.2 mmol). The reaction was heated in an oil bath under reflux for 17 h. The solution was concentrated in vacuo to 1 mL, and then the residue was dissolved in Et₂O, which caused formation of a solid that was removed by filtration. After the filtrate was washed with 1 N NaOH (0.5 mL), the organic layer was dried (Na₂SO₄) and filtered, and the filtrate was concentrated on a rotary evaporator. Excess triethyl phosphite was removed using high vacuum to afford phosphonate 15 (370 mg, 69%) as a colorless oil. Both the ¹H and ³¹P NMR spectra were in agreement with data reported for compound 15 prepared by a traditional Arbuzov sequence.¹⁰

Attempted Preparation of 7-[(E)-2-[(7R,8aR,10aR)-4-Methoxy-7-(methoxymethoxy)-8,8,10a-trimethyl-6,7,8a,9-tetrahydro-5H-xanthen-2-yl]vinyl]-3-(2-methyl-1,3-dioxolan-2-yl)chromen-2-one (33)

To a flame-dried round-bottom flask containing the phosphonate 15 (78 mg, 0.17 mmol) in THF (1 mL) at 0 °C was added NaH (60% dispersion in oil, 10 mg, 0.25 mmol). To the stirring solution was added the aldehyde 30 (8.6 mg, 26 μmol) in THF (2 mL). The reaction was allowed to warm to rt naturally and then was quenched by addition of H₂O. The organic compounds were extracted into EtOAc, dried (Na₂SO₄), and filtered, and the filtrate was concentrated in vacuo. The desired stilbene 33 could not be detected in the resulting material.

[4-Bromo-3,5-bis(methoxymethoxy)phenyl]methoxy tert-Butyldimethylsilane (34)

To an oven-dried and argon-purged round-bottom flask containing alcohol 25 (1.3 g, 3.4 mmol) in anhydrous CH₂Cl₂ (200 mL) was added imidazole (470 mg, 6.9 mmol) followed by TBSCl (560 mg, 3.8 mmol), and the reaction was allowed to stir at rt for 14 h. After the reaction was quenched by addition of water, the aqueous layer was extracted with CH₂Cl₂. The combined organic layers were washed with brine, dried (Na₂SO₄), and filtered through a pad of Celite, and the filtrate was concentrated under reduced pressure to afford the silyl ether 34 as a yellow oil (1.4 g, 97%): ¹H NMR (400 MHz, CDCl₃): δ 6.84 (s, 2H), 5.24 (s, 4H), 4.68 (s, 2H), 3.52 (s, 6H), 0.94 (s, 9H), 0.10 (s, 6H). ¹³C{¹H} NMR (75 MHz, CDCl₃): δ 158.5 (2 C), 144.4 (C), 107.3 (2 CH), 103.5 (C), 94.7 (2 CH₂), 64.9 (CH₂), 56.1 (2 CH₃), 26.1 (3 CH₃), 18.6 (C), −5.1 (2 CH₃).⁴¹

4-(((tert-Butyldimethylsilyl)oxy)methyl)-2,6-bis(methoxymethoxy)benzaldehyde (35)

An oven-dried round-bottom flask containing aryl bromide 34 (5.6 g, 13 mmol) in Et₂O (150 mL) was cooled to −78 °C for 20 min. To the solution was added n-BuLi (7.4 mL, 18 mmol, 2.4 M). Immediately after the addition was complete, anhydrous DMF (1.4 mL, 18 mmol) was added dropwise and the reaction was allowed to stir and warm to rt overnight. After the reaction was quenched by addition of saturated NH₄Cl (50 mL), the organic compounds were extracted into Et₂O (3 × 50 mL). The combined organic layers were dried (Na₂SO₄), the solids were removed by filtration, and the filtrate was concentrated on a rotary evaporator. Final purification was achieved by ISCO normal-phase auto-chromatography (0–5% EtOAc in hexanes), which gave aldehyde 35 as a yellow oil (2.61 g, 53%). Both the ¹H and ¹³C NMR spectra match the literature data for material prepared by a different route.⁴²¹H NMR (300 MHz, CDCl₃): δ 10.34 (s, 1H), 6.70 (s, 2H), 5.09 (s, 4H), 4.56 (s, 2H), 3.32 (s, 6H), 0.80 (s, 9H), 0.05 (s, 6H).

tert-Butyl-[[4-(1,3-dioxolan-2-yl)-3,5-bis(methoxymethoxy)phenyl]methoxy]dimethylsilane (36)

A round-bottom flask containing aldehyde 35 (680 mg, 1.8 mmol), ethylene glycol (660 μL, 11 mmol), and PPTS (110 mg, 370 μmol) in benzene (50 mL) was fitted with a Dean–Stark trap and heated in an oil bath under reflux. The reaction progress was monitored periodically by thin-layer chromatography. After 1 h, the reaction was allowed to cool to rt, quenched by addition of saturated NaHCO₃ (5 mL), and extracted with EtOAc (3 × 20 mL). The combined organic layers were dried (Na₂SO₄), the solid was removed by gravity filtration, and the resulting filtrate was concentrated on a rotary evaporator to afford acetal 36 (720 mg, 96%): ¹H NMR (400 MHz, CDCl₃) 6.74 (2H, s), 6.40 (1H, s), 5.09 (4H, s), 4.63 (2H, s), 4.11 (2H, m), 3.89 (2H, m), 3.39 (6H, s), 0.89 (9H, s), 0.03 (6H, s); ¹³C NMR (101 MHz, CDCl₃) 157.4 (C), 145.0 (C), 114.1 (2 CH), 106.5 (CH), 98.9 (2 C), 94.7 (2 CH₂), 65.6 (2 CH₂), 64.4 (CH₂), 56.1 (2 CH₃), 26.2 (3 CH₃), 18.4 (C), −5.4 (2 CH₃). HRMS (ESI) m/z: [M + H]⁺ calcd for C₂₀H₃₅O₇Si, 417.2147; found, 415.2145.

[4-(1,3-Dioxolan-2-yl)-3,5-bis(methoxymethoxy)phenyl]methanol (37)

To a flask containing the silyl ether 36 (2.5 g, 6.0 mmol) in THF (150 mL) was added TBAF (1.0 M in THF, 6.0 mL, 6.0 mmol), and the solution was allowed to stir at 0 °C and naturally warm to rt over 1 h. The reaction was quenched by addition of water, and the organic compounds were extracted into EtOAc (3 × 100 mL), washed with brine, and dried (Na₂SO₄). The solids were removed by gravity filtration, and the filtrate was concentrated on a rotary evaporator to afford alcohol 37 as a pale yellow solid (1.7 g, 94%): ¹H NMR (400 MHz, CDCl₃) 6.81 (2H, s), 6.48 (1H, s), 5.20 (4H, s), 4.57 (2H, s), 4.24–4.19 (2H, m), 4.04–4.01 (2H, m), 3.50 (6H, s); ¹³C NMR (101 MHz, CDCl₃): δ 157.3 (2 C), 144.2 (C), 114.8 (C), 106.9 (2 CH), 98.5 (CH), 94.7 (2 CH₂), 66.1 (2 CH₂), 65.4 (CH₂), 56.3 (2 CH₃). HRMS (ESI) m/z: [M + H]⁺ calcd for C₁₄H₂₁O₇, 301.1282; found, 301.1280.

4-(1,3-Dioxolan-2-yl)-3,5-bis(methoxymethoxy)benzaldehyde (38)

To a flame-dried and argon-purged round-bottom flask containing alcohol 37 (210 mg, 690 μmol) in anhydrous CH₂Cl₂ (20 mL) was slowly added manganese dioxide (1.5 g, 14 mmol). The resulting mixture was allowed to stir at rt for 26 h. The mixture then was filtered through a bed of Celite, and the filtrate was concentrated to afford aldehyde 38 (180 mg, 86%): ¹H NMR (400 MHz, CDCl₃): δ 9.89 (s, 1H), 7.29 (s, 2H), 6.50 (s, 1H), 5.24 (s, 4H), 4.23–4.20 (m, 2H), 4.04–4.00 (m, 2H), 3.49 (s, 6H); ¹³C NMR (101 MHz, CDCl₃): δ 191.7 (CH), 158.0 (2 C), 138.1 (C), 121.5 (C), 109.7 (2 CH), 98.1 (CH), 94.5 (2 CH₂), 66.5 (2 CH₂), 56.6 (2 CH₃). HRMS (ESI) m/z: [M + H]⁺ calcd for C₁₄H₁₉O₇, 299.1125; found, 299.1127.

(2R,4aR,9aR)-7-[(E)-2-[4-(1,3-Dioxolan-2-yl)-3,5-bis(methoxymethoxy)phenyl]vinyl]-5-methoxy-2-(methoxymethoxy)-1,1,4a-trimethyl-3,4,9,9a-tetrahydro-2H-xanthene (39)

To a flame-dried and argon-purged round-bottom flask containing the phosphonate 15 (116 mg, 250 μmol) and the aldehyde 38 (61 mg, 0.21 mmol) in THF (12 mL) at 0 °C was added dropwise NaHMDS (1.0 M in THF, 680 μL, 680 μmol), and the mixture was allowed to stir at 0 °C for 32 h. The reaction was quenched by a dropwise addition of saturated NH₄Cl to reach a pH of 8, and the organic compounds were extracted into EtOAc (3 × 20 mL). The combined organic layers were washed with brine and dried (MgSO₄), the solids were removed by filtration, and the filtrate was concentrated on a rotary evaporator. Final purification was achieved by column chromatography (15–100% EtOAc in hexanes) to afford the recovered phosphonate 15 (98 mg, 83%) and the desired stilbene 39 as a fluorescent yellow oil (25 mg, 16%): ¹H NMR (400 MHz, CDCl₃): δ 6.98 (d, J = 14.8 Hz, 1H), 6.92 (s, 2H), 6.90–6.84 (m, 3H), 6.47 (s, 1H), 5.22 (s, 4H), 4.77 (d, J = 7.1 Hz, 1H), 4.65 (d, J = 6.4 Hz, 1H), 4.24–4.19 (m, 2H), 4.02–3.99 (m, 2H), 3.89 (s, 3H), 3.51 (s, 6H), 3.41 (s, 3H), 3.28 (dd, J = 11.3, 3.5 Hz, 1H), 2.71 (d, J = 8.6 Hz, 1H), 2.15 (d, J = 12.9 Hz, 1H), 2.02–1.92 (m, 1H), 1.84–1.75 (m, 1H), 1.75–1.69 (m, 1H), 1.65–1.54 (m, 2H), 1.25 (s, 3H), 1.10 (s, 3H), 0.91 (s, 3H); ¹³C NMR (101 MHz, CDCl₃): δ 157.5 (2C), 149.3, 142.9, 140.6, 130.1, 126.1, 122.7, 121.1, 114.8, 109.7, 107.0, 106.8 (2C), 98.8, 96.5, 94.9 (2C), 84.1, 66.0 (2C), 56.3, 56.0, 55.6, 47.0, 38.3, 37.6, 29.8, 27.5, 25.4, 23.1, 19.9, 15.2, 14.2. HRMS (ESI) m/z: [M + H]⁺ calcd for C₃₄H₄₇O₁₇, 615.3164; found, 615.3171.

4-[(E)-2-[(7R,8aR,10aR)-7-Hydroxy-4-methoxy-8,8,10a-trimethyl-6,7,8a,9-tetrahydro-5H-xanthen-2-yl]vinyl]-2,6-dihydroxybenzaldehyde (40)

To the acetal 39 (35 mg, 57 μmol) was added 5 M HCl (5 mL) and anhydrous MeOH (15 mL); the solution was allowed to stir at rt for 69 h, and then was quenched by addition of saturated NaHCO₃ to a pH of 7. The organic compounds were extracted into EtOAc (2 × 25 mL), the combined organic layers were dried (MgSO₄), and the solids were removed by filtration. The filtrate was concentrated to afford the aldehyde 40 as an orange solid (26 mg, 100%): ¹H NMR (400 MHz, CDCl₃): δ 10.27 (s, 1H), 7.05 (d, J = 15.9 Hz, 1H), 6.85 (m, 2H), 6.76 (d, J = 15.4 Hz, 1H), 6.47 (s, 2H), 3.87 (s, 3H), 3.44 (d, J = 6.5 Hz, 1H), 2.71 (d, J = 8.6 Hz, 2H), 2.18–2.07 (m, 2H), 1.92–1.80 (m, 1H), 1.73–1.49 (m, 2H), 1.24 (s, 3H), 1.09 (s, 3H), 0.87 (s, 3H); ¹³C NMR (101 MHz, CDCl₃): δ 193.1 (CH), 149.1 (2 C), 148.1, 143.7, 133.2, 128.0, 125.1 (2 C), 123.0, 121.7, 109.5, 107.4, 105.2, 78.4, 77.5, 56.1, 46.8, 38.5, 37.6, 29.7, 28.2, 27.3, 23.1, 19.9, 14.3. HRMS (ESI) m/z: [M – H]^– calcd for C₂₆H₂₉O₆, 437.1964; found, 437.1965.

7-[(E)-2-[(7R,8aR,10aR)-7-Hydroxy-4-methoxy-8,8,10a-trimethyl-6,7,8a,9-tetrahydro-5H-xanthen-2-yl]vinyl]-3-acetyl-5-hydroxychromen-2-one (41)

To a flame-dried round-bottom flask containing the aldehyde 40 (19 mg, 41 μmol) in anhydrous MeOH (2 mL) were added ethyl acetoacetate (27, 5.2 μL, 41 μmol) and piperidine (2.0 μL, 20 μmol) and the sides of the flask were washed with 1 mL of anhydrous MeOH. The solution was allowed to stir in a foil-covered flask at rt for 75 h, and then the reaction was quenched by addition of H₂O (10 mL). The organic compounds were extracted into CH₂Cl₂ (3 × 20 mL), the combined organic layers were dried (Na₂SO₄), and the solids were removed by filtration. The filtrate was concentrated on a rotary evaporator, and the resulting material was purified by column chromatography (50–100% EtOAc in hexanes). Final purification was achieved by washing the solid with pentane (3 × 2 mL) to afford coumarin 41 as a fluorescent orange solid (22 mg, 100%): ¹H NMR (400 MHz, CD₃CN): δ 8.66 (s, 1H), 7.27 (d, J = 15.9 Hz, 1H), 7.07–7.01 (m, 3H), 6.94 (s, 2H), 3.86 (s, 3H), 3.71–3.67 (m, 1H), 2.76 (d, J = 8.8 Hz, 2H), 2.61 (s, 3H), 1.80–1.71 (m, 3H), 1.71–1.65 (m, 2H), 1.22 (s, 3H), 1.09 (s, 3H), 0.87 (s, 3H); ¹³C NMR (151 MHz, CD₃CN): δ 195.3, 169.0, 166.5, 160.8, 159.1, 156.2, 149.4, 148.9, 141.7, 133.2, 124.3, 122.8, 121.9, 121.1, 108.1, 108.0, 107.7, 104.4, 77.1, 76.9, 55.2, 46.5, 38.2, 37.5, 29.2, 26.7, 25.9, 23.4, 19.3, 13.9. HRMS (ESI) m/z: [M + Na]⁺ calcd for C₃₀H₃₂O₇Na, 527.2046; found, 527.2051.

Ethyl 7-Methyl-3-oxo-6-octenoate (42)

To an oven-dried and argon-purged round-bottom flask containing NaH (2.08 g, 51.9 mmol) in THF (400 mL) at 0 °C was added ethyl acetoacetate (27, 6.02 mL, 47.2 mmol), and the solution was stirred for 10 min. To the reaction flask was added dropwise n-BuLi (21.6 mL, 51.9 mmol) followed by a dropwise addition of prenyl bromide (6.00 mL, 51.9 mmol). The reaction was stirred at rt for 20 min and then quenched by addition of saturated NH₄Cl and extracted with Et₂O. The combined organic layers were dried (Na₂SO₄) and filtered, and the filtrate was concentrated on a rotary evaporator. Final purification was achieved by column chromatography (10% EtOAc in hexanes) to afford the β-ketoester 42 as a pale yellow oil (4.69 g, 50%)^. Both the ¹H and ¹³C NMR spectra matched those in the literature.⁴³

5-(Methoxymethoxy)-7-(methoxymethyl)-3-(5-methylhex-4-enoyl)chromen-2-one (43)

To a mortar were added aldehyde 26 (806 mg, 3.56 mmol), the β-ketoester 42 (1.41 g, 7.12 mmol), and piperidine (three drops). The solution was ground with a pestle intermittently over 12 h. Purification was achieved by column chromatography (20–40% EtOAc in hexanes). The desired fractions were combined and heated (oil bath) under reflux in benzene with a Dean–Stark trap overnight. After cooling to rt, the resulting solution was concentrated in vacuo to afford compound 43 (1.12 g, 87%): ¹H NMR (400 MHz, CDCl₃): δ 8.83 (s, 1H), 6.93 (s, 1H), 6.90 (s, 1H), 5.30 (s, 2H), 5.13 (tt, J = 7.2, 1.3 Hz, 1H) 4.47 (s, 2H), 3.49 (s, 3H), 3.41 (s, 3H), 3.12 (t, J = 7.2 Hz, 2H), 2.34 (q, J = 7.2 Hz, 2H), 1.64 (s, 3H), 1.60 (s, 3H); ¹³C{¹H} NMR (100 MHz, CDCl₃): δ 197.7 (C), 159.2 (C), 156.1 (C), 155.6 (C), 147.5 (CH), 142.8 (C), 132.5 (C), 123.0 (CH), 122.3 (C), 109.0 (C), 107.6 (CH), 107.0 (CH), 94.7 (CH₂), 73.8 (CH₂), 58.7 (CH₃), 56.7 (CH₃), 42.6 (CH₂), 25.7 (CH₃), 22.6 (CH₂), 17.7 (CH₃). HRMS (ESI) m/z: [M + H]⁺ calcd for C₂₀H₂₅O₆, 361.1646; found, 361.1624.

Supporting Information Available

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.joc.1c02046.

• ¹H and ¹³C NMR spectra for all new compounds and full tables of the bioassay data (PDF)

The authors declare no competing financial interest.