FIGURE 2.

Surface localization of Fur4 is required for growth in low uracil. (A) Vacuoles from indicated strains expressing Fur4‐mNeonGreen (mNG) were labelled with FM4‐64 prior to confocal imaging. (B) Wild‐type, rcy1∆ and nhx1∆ cells grown to mid‐log phase were then spotted in a 1 in 10 serial dilution onto plates titrated with indicated concentrations of uracil and grown at 30°C for 3 days. (C) Growth of strains from (B) were quantified, asterisks (*) indicate Student t test comparisons of mutants with wild‐type cells, P = <.005. (D) Schematic diagrams showing the predicted effects on Fur4 following different trafficking pathway perturbations. Scale bar = 5 μm