Figure 2.

Ndufa6 knockdown inhibits adipogenesis. Before adipogenic differentiation, OP9 cells were transfected with Ndufa6 siRNA (Ndufa6 KD) or control siRNA (NC), and 24 h later, cells were incubated with 1 mM rosiglitazone to induce adipogenic differentiation. (a) RT–qPCR was used to test the Ndufa6 silencing efficiency in OP9 cells. (b) Oil red O staining was performed after adipogenic differentiation. The lipid droplets were stained red, and nuclei were stained blue by haematoxylin. The scale bar in the figure is 200 μm. The cellular TG content was tested in OP9 cells after adipogenic differentiation (n = 3), and the test results were normalized by the protein content. (c) The relative mRNA levels of adipogenic differentiation marker genes were tested with RT–qPCR (n = 3). Values are presented as the mean ± standard deviation. Statistical analysis was performed by the LSD t-test. * P < 0.05; ** P < 0.01; *** P < 0.005