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. 2021 Nov 26;24(12):103517. doi: 10.1016/j.isci.2021.103517

Figure 2.

Figure 2

GFAT2 mediates cardiomyocyte hypertrophy by protein O-GlcNAcylation

(A) Representative immunoblots of GFAT2, GFAT1, OGT, OGA, protein O-GlcNAcylation, and GAPDH in NRVMs treated with indicated small interfering RNA (siRNA) in the presence or absence of ISO (1 μM, 12 h).

(B–F) Quantitative analysis of GFAT2, GFAT1, OGT, OGA, and protein O-GlcNAcylation in NRVMs treated with indicated small interfering RNA (siRNA) in the presence or absence of ISO (1 μM, 12 h) (n = 6).

(G) Cell surface area of NRVMs treated with indicated siRNA in the presence or absence of ISO (1 μM, 24 h) (n = 150 cells in each group). Scale bar, 50 μm. ∗p < 0.05, ∗∗p < 0.01: post hoc Tukey's comparison test.