(A) Experimental protocol.
(B) CTLA-4 expression in CD45.2+
Ctla4f/f or control Treg cells in the TME following tamoxifen treatment.
(C and D) Expression of Ki67 (C) and frequency (D) of CTLA-4-sufficient (n = 8) or CTLA-4-deficient (n = 7) CD45.2+ Treg cells in the TME (pooled from two experiments). Fold change as compared to control groups indicated in parentheses. MFI was normalized by the mean of control groups. p values calculated by Mann-Whitney U test.
See also Figure S6.