FIG 3.

Intermediate production and cross-modification of 15-HT by the nonclustered methylglucosylation genes. (A) HPLC analysis showing the production or nonproduction of different intermediate compounds after gene deletions in the OE::tenR mutant of B. bassiana (Bb). (B) Verification of the methylglucosylation genes contributing to the production of compound 1 in B. bassiana. (C) Verification of the cross-modification of 15-HT by M. robertsii (Mr). (D) Substrate feeding assay confirming the conversion of compound 3 to compound 1 by MrGT1. Feeding with compound 4 could not be converted by M. robertsii. (E) Compound conversions by transgenic yeast cells. Compound 2 or 3 was added to the media at a final concentration of 10 μg/ml for 2 days.