Figure 5.

Host cell protein S100A6 promoted T. gondii invasion

(A) Representative wide-field epifluorescence images (10 × 100) for the COS7 cells transfected with the indicated plasmid and infected by RH-WT. The intracellular parasites are labeled red, and the extracellular parasites are labeled both green and red to appear as yellow (scale bars, 10μm

B) Verification of S100A6 overexpression in COS7 cells by WB.

(C) Quantitative analysis of T. gondii attachment and invasion efficiency in different treatment groups (presented with parasites/100 host cells). Each experiment was performed in triplicate. Quantification of the mean ± SD and analyzed by t test (∗∗p < 0.05).