TgSAG1 promoted TNF-α secretion in THP-1 cells
(A–C) Transcriptional levels of IL-12p70, IFN-γ, TNF-α in RH-WT or RH-Δsag1 infected THP-1 cells were detected by qRT-PCR.
(D) The secretion of TNF-α in RH-WT or RH-Δsag1 infected THP-1 cells were detected by ELISA. Data were presented as pg/mL.
(E) Verification of TgSAG1 overexpression in RAW264.7 cells by WB.
(F and G) the transcription and secretion of TNF-α in TgSAG1 transfected RAW264.7 cells were detected by qRT-PCR and ELISA, respectively. Data were presented as means ± SD combined from three independent experiments and statistical significance were analyzed by t test (∗∗p < 0.05).