Figure 6.
Evaluation of PCL scaffolds treatments with SEM evaluation in order to determine the impact of treatment on NHDFs attachment and infiltration. The treatments of the 0° architectured PCL scaffolds included: (A) 1X Matrigel™ in DMEM overnight incubation; (B) 1X Matrigel™ in DMEM overnight incubation with NHDFs seeding (100 000 cells/construct); (C) collagen hydrogel (1 mg ml−1) seeding; (D) collagen hydrogel (1 mg ml−1) with NHDFs seeding (100 000 cells/construct); (E) bare PCL scaffold with NHDF seeding (100 000 cells/construct); and (F) 1X Matrigel™ in DMEM overnight incubation and collagen hydrogel (1 mg ml−1) casting with NHDFs seeding (100 000 cells/construct). All tissues were evaluated after 48 h in culture. Scale bars = 500 μm.