KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| p44/42 MAP kinase (phosphorylated Erk1/2) antibody | Cell Signaling | Cat# 9101, RRID:AB_331646 |
| Phospho-FAK (Tyr397) (D20B1) Rabbit mAb antibody | Cell Signaling | Cat# 8556, RRID:AB_10891442 |
| Rabbit Anti-GAPDH Monoclonal Antibody, Unconjugated, Clone 14C10 | Cell Signaling | Cat# 2118, RRID:AB_561053 |
| Chemicals, peptides, and recombinant proteins | ||
| Phorbol-12,13-dibutyrate (PDBu) | EMD Millipore | #524390 |
| Anisomycin | Sigma-Aldrich | A9789 |
| UK14304 | Sigma-Aldrich | U104 |
| Yohimbine | Sigma-Aldrich | Y3125 |
| Gefitinib | Cayman | #13166 |
| Ionomycin | Peprotech | #5608212 |
| Jasplakinolide | Cayman | #11705 |
| Latrunculin B | Enzo Life Sciences | BML-T110–0001 |
| PF562271 | AdipoGen | SYN-1064 |
| ZSTK474 | Cell Signaling | #13213 |
| Dasatinib | Cayman | #11498 |
| GDC-0994 | APExBIO Technology | B5817 |
| LY2584702 | Selleck | S7698 |
| BAPTA-AM | Selleck | S7534 |
| Y27632 | Enzo Life Sciences | ALX-270–333 |
| EGF | Sigma-Aldrich | E9644 |
| 2-Deoxyglucose (2-DG) | Sigma-Aldrich | D8375 |
| Experimental models: Cell lines | ||
| Human: A6L | L. Wood Lab (JHU) | N/A |
| Human: HEK293T | C. Hung Lab (JHU) | N/A |
| Human: HeLa | M. Iijima Lab (JHU) | N/A |
| Human: MCF7 | C. Hung Lab (JHU) | N/A |
| Human: SiHa | C. Hung Lab (JHU) | N/A |
| Human: U2OS | ATCC | HTB-96 |
| Human: U87MG | M. Iijima Lab (JHU) | N/A |
| Oligonucleotides | ||
| Primer for ERKKTR (forward): CAAgtcgacATGAAGGGCCGAAAGCCTC | This paper | N/A |
| Primer for ERKKTR (reverse): CAAggatccccGGATGGGAATTGAAAGCTGGACT | This paper | N/A |
| Primer for p38KTR (forward): CAActcgagATGCGTAAGCCAGATCTCCG | This paper | N/A |
| Primer for p38KTR (reverse): CAAggatccccGCTGGACTGGAGGGTCAG | This paper | N/A |
| Primer for JNKKTR (forward): CAActcgagATGAGTAACCCTAAGATCCTAAAACAGAG | This paper | N/A |
| Primer for JNKKTR (reverse): CAAggatccccGCTGGACTGGAGGGTCAG | This paper | N/A |
| Recombinant DNA | ||
| AMPKAR | Tsou et al., 2011 | Addgene plasmid #35097 |
| AMPKAR-EV | Konagaya et al., 2017 | Addgene plasmid #105241 |
| cyto-ABKAR | Miyamoto et al., 2015 | Addgene plasmid #61510 |
| LAMP1-ABKAR | Miyamoto et al., 2015 | Addgene plasmid #65068 |
| CaNAR2 | Mehta et al., 2014 | Addgene plasmid #64728 |
| ER-CaNAR2 | Mehta et al., 2014 | Addgene plasmid #64732 |
| PM-CaNAR2 | Mehta et al., 2014 | Addgene plasmid #64730 |
| GCaMP6S | Chen et al., 2013 | Addgene plasmid #40753 |
| GCaMP6S-PM | Tsai et al., 2014 | Addgene plasmid #52228 |
| EKAR | Harvey et al., 2008 | Addgene plasmid #18679 |
| Nuc-EKAR | Harvey et al., 2008 | Addgene plasmid #18681 |
| Lyn-EKAR | This paper | N/A |
| ERKKTR | Regot et al., 2014 | Addgene plasmid #59150 |
| Cyto-FAK | Seong et al., 2011 | Addgene plasmid #78300 |
| Lyn-FAK | Seong et al., 2011 | Addgene plasmid #78299 |
| Src | Ouyang et al., 2008 | Addgene plasmid #78302 |
| Gαi1 | van Unen et al., 2016 | Addgene plasmid #69623 |
| Gαi2 | van Unen et al., 2016 | Addgene plasmid #69624 |
| Gαi3 | van Unen et al., 2016 | Addgene plasmid #69625 |
| JNKAR | Fosbrink et al., 2010 | Addgene plasmid #61625 |
| JNKKTR | Regot et al., 2014 | Addgene plasmid #59151 |
| p38KTR | Regot et al., 2014 | Addgene plasmid #59152 |
| PH-AKT | Watton and Downward, 1999 | N/A |
| AKTAR2 | Zhou et al., 2015 | Addgene plasmid #64932 |
| CKAR | Violin et al., 2003 | Addgene plasmid #14860 |
| PM-CKAR | Violin et al., 2003 | Addgene plasmid #14862 |
| RhoA-2G | Fritz et al., 2013 | Addgene plasmid #40176 |
| Rac1-2G | Fritz et al., 2015 | Addgene plasmid #66110 |
| Cdc42–2G | Martin et al., 2016 | Addgene plasmid #68814 |
| LifeAct | Riedl et al., 2008 | Addgene plasmid #54610 |
| Raichu-HRas | Komatsu et al., 2011 | N/A |
| Raichu-KRas | Komatsu et al., 2011 | N/A |
| Raichu-Rras | Takaya et al., 2007 | N/A |
| BKAR | Kunkel et al., 2005 | Addgene plasmid #14875 |
| TORCAR | Zhou et al., 2015 | Addgene plasmid #64927 |
| Prin-BRaf | Terai and Matsuda, 2006 | N/A |
| Prin-CRAF | Terai and Matsuda, 2005 | N/A |
| EV-RSK | Komatsu et al., 2011 | N/A |
| EV-ROCK | Li et al., 2017 | N/A |
| EV-S6K | Komatsu et al., 2011 | N/A |
| EV-EGFR (PicchuEV(CrkII)) | Komatsu et al., 2011 | N/A |
| Syk biosensor | Xiang et al., 2011 | Addgene plasmid #125729 |
| pEGFP-N1 | CloneTech | N/A |
| EBFP2-Nucleus-7 | gift from Michael Davidson | Addgene plasmid #55249 |
| H2B-TagRFP | gift from Philipp Keller | Addgene plasmid #99271 |
| pmCherry-NLS | Micutkova et al., 2012 | Addgene #39319 |
| mCardinal-H2B-C-10 | Chu et al., 2014 | Addgene plasmid #56162 |
| mCardinal-N1 | Chu et al., 2014 | Addgene #54590 |
| piRFP702-N1 | Shcherbakova and Verkhusha, 2013 | Addgene plasmid #45456 |
| piRFP682-N1 | Shcherbakova and Verkhusha, 2013 | Addgene plasmid #45459 |
| pNLS-iRFP670 | Shcherbakova and Verkhusha, 2013 | Addgene plasmid #45466 |
| mNeptune2-C1 | gift from Michael Davidson | Addgene plasmid #54836 |
| mNeptune2.5 | Chu et al., 2014 | Addgene plasmid #51310 |
| mPlum-C1 | Kremers et al., 2009 | Addgene plasmid #54839 |
| RDSmCherry1 | Shen et al., 2017 | Addgene plasmid #89987 |
| LSS-mKate2-C1 | Piatkevich et al., 2010 | Addgene plasmid #31869 |
| mStrawberry-N1 | Shaner et al., 2004 | Addgene #54644 |
| pDONR221 | Invitrogen | #12536017 |
| pLex307 | gift from David Root | Addgene plasmid #41392 |
| pMD2.G | gift from Didier Trono | Addgene plasmid #12259 |
| psPAX2 | gift from Didier Trono | Addgene plasmid #12260 |
| Software and algorithms | ||
| Zen Microscopy Software | Zeiss | https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
| ImageJ/Fiji | Schindelin et al., 2012; Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| Python | Python Software Foundation | https://www.python.org |
| Keras | Chollet, 2018 | https://keras.io/ |
| TensorFlow | Abadi et al., 2015 | https://www.tensorflow.org/ |
| Dropout | Srivastava et al., 2014 | N/A |
| RMSprop | Hinton et al., 2012 | N/A |
| Adam | Kingma and Ba, 2014 | N/A |
| Glorot Initializer | Glorot and Bengio, 2010 | N/A |
| MATLAB | MathWorks | https://www.mathworks.com/products/matlab.html |