In the original article, there was a mistake in Figure 4 as published. The western blot of reference gene (GAPDH) in hypoxia group in Figure 4D was incorrectly used and plotted in this figure. However, this mistake doesn’t affect the results of experiment and understanding of our research on purpose. The corrected Figure 4 appears below.
Figure 4.
ZEB-1AS1 transcription is regulated by HIF-1α during hypoxia medium. (A) A putative hypoxia-responsive element (HRE) was found in the promoter region of ZEB1-AS1. (B) The expression levels of ZEB1-AS1 (upper) and HIF-1α protein (lower) in BXPC-3/PANC-1 cells were measured after being cultured during normoxia, hypoxia (1%O2), or CoCl2 (concentration of 100 µM under 48 h) at the mRNA and protein levels by qRT-PCR and Western blot analysis, respectively. (C, D) After knockdown of HIF-1α with siRNA, the expression of ZEB1-AS1 was evaluated by qRT-PCR in BXPC-3 and PANC-1 cells under normoxia or hypoxia (upper). Lower diagrams indicated HIF-1α protein levels by Western blot analysis. (E) ChIP assays with anti-HIF-1α antibody were performed to affirm the binding between HIF-1α and HRE of ZEB1-AS1 promoter region in PANC-1 cells under normoxia or hypoxia condition. (F) After being cultured in hypoxia or normoxia, ChIP assays with anti-Pol II antibody were performed to ascertain the binding capacity between Pol II and ZEB1-AS1 promoter region in PANC-1 cells. (G) After knockdown of HIF-1α, the expression of ZEB1-AS1 was shown by FISH assays in PANC-1 cells during normoxia and hypoxia condition. (H) Wild-type ZEB1-AS1 promoter-containing pGL3 reporter vector (WT) or mutant-type ZEB1-AS1 promoter-containing pGL3 reporter vector (MUT) of ZEB1-AS1 promoter sequence firefly luciferase reporter activity in PANC-1 cells transfected with siNC or siHIF-1α and cultured under normoxia or hypoxia conditions were assessed by Renilla luciferase reporter assays after 48 h All data were presented as means ± SD of at least three independent experiments. Values are significant at *p ≤ 0.05 and **p ≤ 0.01 as indicated.
The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.
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