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. 2022 Jan 1;12(1):143–166. doi: 10.7150/thno.57234

Figure 3.

Figure 3

hPSC-EMSCs dampen the activation of glial and microglial cells more effectively than hUC-MSCs in HIE rats. The third day after HI (PN 10), 2 × 105 hUC-MSCs or hPSC-EMSCs (derived from H7 or iPSCs) in 5 μL of PBS or vehicle were infused into the ipsilateral hemisphere. A) Representative immunofluorescent staining images of GFAP-, vimentin- and Iba-1-stained HIE rats in the cortex. Focused area in the brain cortex is shown with a magnified view(inset) demonstrating GFAP or vimentin or Iba-1 positive cells (green, arrowhead). The average intensity of GFAP, vimentin and Iba-1 was measured in three coronal sections (6 dpi, bregma -1.7, -1.8 and -1.9 mm; 10 dpi, bregma -2.0, -2.1 and -2.2 mm), scale bar: 100 µm. Quantification data represent mean ± SEM (6 dpi, sham: n = 3, PBS: n = 6, hUC-MSCs: n = 5, hPSC-EMSCs: n = 6; 10 dpi, sham: n = 3, PBS: n = 5, hUC-MSCs: n = 5, hPSC-EMSCs: n = 6). *, **, ***, **** represent p < 0.05, 0.01, 0.001 and 0.0001 respectively by Tukey's post-hoc test when statistical significance by One-way ANOVA (p < 0.05) was obtained.