Abstract
By immunodiffusion and immunoelectrophoresis tests in agarose serological cross-reactions were demonstrated between Yersinia enterocolitica type IX and Brucella strains from four species (Brucella abortus, Brucella melitensis, Brucella suis and Brucella neotomae). No qualitative differences between these strains in their tendencies to cross-react with Yersinia enterocolitica type IX were observed. Brucella canis and Brucella ovis, which have nonsmooth colonial morphology, gave no demonstrable cross-reaction with Yersinia enterocolitica type IX.
The results of absorption tests and qualitative staining reaction of the obtained precipitation lines suggest that the antigenic determinants common to Brucella and Yersinia enterocolitica type IX seemed to be associated with the outer layer and in the lipopolysaccharide complex of the respective bacteria. By immunodiffusion and immunoelectrophoresis it was possible to identify in hyperimmune sera those antibodies that derive from Brucella and Yersinia enterocolitica type IX.
Keyword: serological cross-reaction, Brucella species, Yersinia enterocolitica serotype IX, immunodiffusion, immunoelectrophoresis
Sammanfattning
Immunodiffusion och immunoelektrofores.
Med hjalp av immunodiffusion och immunoelektrofores i agarose har en serologisk korsreaktion påvisats mellan Yersinia enterocolitica typ IX och Brucella stammar från 4 olika species (Brucella abortus, Brucella melitensis, Brucella suis och Brucella neotomae). Några kvalitativa skillnader för dessa stammar med avseende på deras benägenhet att korsreagera med Yersinia enterocolitica typ IX har inte kunnat påvisas. Brucella canis och Brucella ovis, som är kolonimorfologiskt av R-typ, har icke givet någon påvisbar korsreaktion med Yersinia enterocolitica typ IX.
Resultaten från gjorda absorptionsförsök och kvalitativa färgningsreaktioner av erhållna precipitationslinjer tyder på att de gemensamma antigena determinanterna för Brucella och Yersinia enterocolitica typ IX torde finnas hos respektive bakteriecellers ytskickt och i deras lipopolysackaridkomplex. Immunodiffusion och immunoelektrofores visar en möjlighet att i hyperimmunsera kunna identifiera de antikroppar som härrör från Brucella respektive Yersinia enterocolitica typ IX.
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