Figure 2.
CCl2 alters the viscoelastic properties of human monocytes, measured with AFS
(A) Magnified image of the flow chamber showing a typical field of view in experiments. Tens of beads on top of the monocytes are individually tracked.
(B) Further magnified image of the typical field of view showing single monocytes with beads attached on top.
(C) Typical trace (creep compliance) of a monocyte in response to the application of a constant force (3.5 nN) at physiological temperature and after exposure to CCL2.
(D) Schematic representations of the KV and SLL model.
(E) CCL2 induces a significant increase in the average elastic modulus Em of monocytes (p < 0.0001).
(F) CCL2 induces a significant increase in the viscosity η of monocytes (p < 0.0001).
(G) CCL2 significantly increases the average elastic modules Ea associated with the cytoskeleton (p < 0.0001).
(H) CCL2 significantly increases the average viscosity ηa associated with the cytoskeleton (p <0.0001).
(I) The average background viscosity ηc of monocytes significantly increases upon stimulation by CCL2 (p <0.0001). Distributions plotted using Origin-Lab (2019b). p-values calculated using Mann-Whitney test.