Critical Commercial Assays |
|
CD14MicroBeads, human for 1×109 total cells |
MiltenyiBiotec |
Cat# 130-050-201 |
AutoMACSpro |
MiltenyiBiotec |
Cat# 130-092-545 |
AutoMACS columns |
MiltenyiBiotec |
Cat# 130-021-101 |
autoMACSRunning Buffer – MACS Separation Buffer |
MiltenyiBiotec |
Cat# 130-091-221 |
autoMACS Washing Solution |
MiltenyiBiotec |
Cat# 130-092-987 |
MACS SmartStrainers (30 μm) |
MiltenyiBiotec |
Cat# 130-098-458 |
|
Other |
RPMI 1640 Medium, GlutaMAXSupplement (No Hepes) |
ThermoFisher Scientific |
Cat# 61870036 |
Penicillin Streptomycin |
ThermoFisher Scientific |
Cat# 15140122 |
FetalBovine Serum |
ThermoFisher Scientific |
Cat# 26140079 |
Ficoll-Paque PLUS |
Sigma-Aldrich |
Cat# GE17-1440-02 |
Greiner LeucoSep-tubes |
Sigma-Aldrich |
Cat# Z642843 |
ConcanavalinA from Canavaliaensiformis
|
Sigma-Aldrich |
Cat# C5275 |
Non-functionalized silica microspheres 4.0μm |
Bangs Laboratories, Inc. |
Cat# SS05002 |
Silica Particles, 5.0% w/v,6.0-8.0 μm |
Spherotech, Inc. |
Cat# SIP-60-10 |
Phosphate Buffered Saline 1x |
Sigma-Aldrich |
Cat# D8537 |