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. Author manuscript; available in PMC: 2021 Dec 22.
Published in final edited form as: Oncogene. 2021 Mar 8;40(14):2553–2566. doi: 10.1038/s41388-020-01599-z

Fig. 7. RGS12 PDZ peptides inhibit osteosarcoma formation and lung metastasis.

Fig. 7.

a Soft agar. Colony formation ability analysis after PDZ or PDZ mutated peptide treatment for 3 weeks in SaOS2 cells. b Immunofluorescent staining of RGS12 and Ezrin in shScramble and shRGS12 cells after co-culture of PDZ peptides and matrigel for 7 days. c, d PDZ peptides inhibited YAP nuclear translocation by increasing the phosphorylation level of YAP in SaOS2 cells. e Representative X-ray images of tumor-bearing legs after intratibial injection with shRGS12 cells mixed with 0 or 100 nm PDZ peptides. f Kaplan-Meier survival analysis indicating overall survival of mice injected with shRGS12 cells mixed with 0 or 100 nM of PDZ peptides. g Representative micro-CT images of tumor-bearing legs after intratibial injection with shRGS12 cells mixed with 0 or 100 nM of PDZ peptides at Day 35. The white arrow indicates the tumor in the bone. h Representative images of HE-stained lung sections of mice injected with shRGS12 cells mixed with 0 or 100 nM of PDZ peptides at Day 35. Scale bar, 100 μm. Error bars were the means ± standard error of the mean (SEM) of triplicates from a representative experiment. **P < 0.01.