Table 3.
Primers used in real-time quantitative PCR (qPCR) for the relative quantification of the tRFs in all samples.
| Amplified Molecule | Primer Sequence (5′→3′) | Direction | Length (nt 1) | Tm (°C) |
|---|---|---|---|---|
| i-tRF-ProTGG | GTTGGTCTAGGGGTATGATTCTCGGA | Forward | 26 | 62 |
| i-tRF-GluCTC | GTCTAGTGGTTAGGATTCGGCGA | 23 | 61 | |
| i-tRF-HisGTG | TGATCGTATAGTGGTTAGTACTCTGCG | 27 | 59 | |
| i-tRF-GlyGCC | GAGGCCCGGGTTCGATTC | 18 | 62 | |
| i-tRF-PheGAA | TTTAGACGGGCTCACATCACC | 21 | 59 | |
| 3’-tRF-LeuAAG/TAG | ATCCCACCGCTGCCACCA | 18 | 66 | |
| SNORD43 | ACTTATTGACGGGCGGACA | 19 | 59 | |
| SNORD48 | TGATGATGACCCCAGGTAACTCT | 23 | 59 | |
| Universal reverse | GCGAGCACAGAATTAATACGAC | Reverse | 22 | 56 |
1 Nucleotides.