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. 2021 Nov 25;11(12):1763. doi: 10.3390/biom11121763

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Detection of Rab7 and cathepsin D in the processing kinetics of M. tuberculosis in 5.5 and 30 mM of glucose. MDMs were infected with M. tuberculosis previously stained for one hour with PKH67 green fluorescent and incubated at 37 °C, 5% CO₂ in a time kinetics (60, 120, and 480 min). (a) Rab 7 (red) and (b) cathepsin D (magenta) quantification of mean fluorescence intensity from at least 100 cells from each condition (5.5 mM and 30mM of glucose) in the kinetic sequence. A confocal microscopy image representative of each condition is shown. Data were analyzed using two-way ANOVA and the differences were considered significant when *** p < 0.0001; from four independent experiments. Confocal image 60×, 1 zoom. (c) Representative image of a reconstruction of Z sections (15 micrometer) and stack of each section of Mtb PKH67 green fluorescent (green), Rab 7 (red), cathepsin D (magenta).

Detection of Rab7 and cathepsin D in the processing kinetics of M. tuberculosis in 5.5 and 30 mM of glucose. MDMs were infected with M. tuberculosis previously stained for one hour with PKH67 green fluorescent and incubated at 37 °C, 5% CO₂ in a time kinetics (60, 120, and 480 min). (a) Rab 7 (red) and (b) cathepsin D (magenta) quantification of mean fluorescence intensity from at least 100 cells from each condition (5.5 mM and 30mM of glucose) in the kinetic sequence. A confocal microscopy image representative of each condition is shown. Data were analyzed using two-way ANOVA and the differences were considered significant when *** p < 0.0001; from four independent experiments. Confocal image 60×, 1 zoom. (c) Representative image of a reconstruction of Z sections (15 micrometer) and stack of each section of Mtb PKH67 green fluorescent (green), Rab 7 (red), cathepsin D (magenta).