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. 2021 Dec 14;11(12):1873. doi: 10.3390/biom11121873

Figure 5.

Figure 5

Effect of alloxan on LXA4 secretion by RIN cells and its modulation by various PUFAs in vitro. ALX = Alloxan. (A) RIN5F (rat insulinoma) cells were treated with 10 µg/mL PUFAs ± ALX (6 mM) for 1 h. The LXA4 was estimated in the supernatant of the cell cultures. All the above sets of experiments were done in triplicate on two separate occasions (n = 6) and values are expressed as mean ± SEM. * p ≤ 0.05 compared to untreated control, # p ≤ 0.05 compared to ALX. (B) RIN5F cells were treated with 15 µg/mL of PUFAs ± ALX (6 mM) for 1 h. The LXA4 was estimated in the supernatants of the cell cultures. All the above sets of experiments were done in triplicate on two separate occasions (n = 6) and values are expressed as mean ± SEM. * p ≤ 0.05 compared to untreated control, # p ≤ 0.05 compared to alloxan (ALX). It is seen that at a 10-µg/mL dose of EPA and DHA treatment there was no increase in LXA4 secretion by RIN5F cells in vitro in the presence of ALX (6 mM) (A). However, when RIN5F cells were supplemented with 15 µg/mL of EPA and DHA, there was a significant increase LXA4 secretion, even in the presence of ALX (6 mM). These data are taken from [13].