Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 15;11(12):1884. doi: 10.3390/biom11121884

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Overproduced Hsp104 disassembles Sup35 prion aggregates, but not fibrils. Hsp104 was either endogenous, or produced from a multicopy plasmid under control of the native promoter (~30-fold excess). (A) Sup35 aggregates of yeast lysates fractionated by centrifugation. Sup35PS, [PSI⁺_PS1], and [PSI⁺_PS2] relate to Sup35 with prion domain from yeast P. methanolica and M and C domains from S. cerevisiae and its two prion variants. (B) SDD-AGE of Sup35 prion fibrils. (A,B) Western blot staining for Sup35NM. Data reproduced from previous research [107] (A), [44] (B).