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. 2021 Dec 6;10(12):3424. doi: 10.3390/cells10123424

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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GEN ameliorated DEX-induced cholesterol accumulation in MC3T3-E1 cells. (A) MTT assays were conducted to investigate the effects of cholesterol on cell viability. (B) The level of intracellular cholesterol after administration with exogenous cholesterol. (C–E) The protein expression of RUNX2 and OPN in CHO (50 μM)-treated MC3T3-E1 cells was determined by western blot. (F) The level of the total intracellular cholesterol was measured by the ELISA kit. All experiments were implemented separately in triplicate. * p < 0.05; ** p < 0.01. OIM, osteogenic induction medium; CHO, cholesterol.