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. 2021 Dec 6;10(12):3424. doi: 10.3390/cells10123424

Figure 5.

Figure 5

GEN promoted ABCA1-mediated cholesterol metabolism in a GLP-1R-dependent manner. (A,B) The in vivo immunohistochemical examination of GLP-1R expression was conducted. (C,D) The protein expression of GLP-1R was determined by western blot in DEX-treated MC3T3-E1 cells. (E) The ALP staining and the Alizarin Red S staining assay were performed in EX-treated MC3T3-E1 cells (×100 magnification). (F) The level of the total intracellular cholesterol was measured using the ELISA kit. (G–K) The protein expression of GLP-1R, ABCA1, RUNX2, and OPN was detected by western blot. All experiments were implemented separately in triplicate. * p < 0.05; ** p < 0.01. NC, negative control; 50 mg/kg, Dex + 50 mg/kg GEN; 100 mg/kg, Dex + 100 mg/kg GEN; EX, Exendin9-39.