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Pneumolysin (PLY) induces neutrophil activation. Human neutrophils were treated with PLY (100 ng/mL, 30 min) and cell surface expression (MFI) of (A) CD11b or (B) CD62L was assessed using flow cytometry. (C) Elastase activity was determined in cell culture supernatants. (D) ROS production was measured using a luminol based kinetic assay. Bars represent area under the curve (AUC) values calculated from kinetic curves. n = 5–9. * p < 0.05, ** p < 0.01, *** p < 0.001, t-test.
