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A live/dead cell assay after treatment of HSC-3 cells with the OI extract for 16 h. (A) Wild type: most of the cells showed green fluorescence, denoting viable cells stained with calcein-AM. (B) OI (50 μg/mL)-treated cells showed cytotoxic activity, represented as more red dead cells stained with Ethidium Bromide. (C) Cisplatin (15 ng/mL) treatment also showed more red cells, indicating dead cells due to the cytotoxic activity of cisplatin.
