Figure 5.

ZIKV INMI1 NS4B blocks STAT2 nuclear transport. (a) Immunofluorescence of HEK293T cells transfected with empty vector (EV) or ZIKV NS2A (row 3) stimulated with IFN-α. FLAG (green) and P-STAT2 (red) signals were detected; nuclei (blue) were stained with Hoechst; scale bar, 10 μm; (b) quantification was performed counting positive cells for nuclear P-STAT1 (red signal) in EV and NS4B transfected cells; 6–10 fields per each condition, from a total of 3 biologically independent experiments, were analyzed; cells count was performed using Cell Counter plugin of ImageJ. **** p <  0.0001 values are reported, as obtained by unpaired t test.