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Trypsin promotes PDCoV to acquire agglutinating sialic acid activity. We detected the level of PDCoVT- (A,D,G), PDCoVT-+trypsin (B,E,H), and PDCoVT-+trypsin- (C,F,I) attached on ST cells and LLC-PK1 cells before and after NA treatment by plaque assay and WB. Differences were considered significant at * p < 0.05, ** p < 0.01; ns: differences were not significant; t-test.
