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Protease expression at mRNA and protein level during recovery. (a) We checked the mRNA expression of serine proteases such as vc0099, vc1200, vca0803 and vc1989 (yaeZ) in a time-dependent manner (4 h, 8 h, 16 h) using dnaK as reference gene. (b) Skim milk plate assay for the detection of protease secretion during recovery from VBNC state. Protease activity on 1% skim milk plate.
