Table 1.
The proportions (%) of genera Colwellia, Cycloclasticus, and Pseudomonas in the bacterial community of seawater (SW), oil-contaminated seawater (SWO), and biostimulated oil-contaminated seawater (SWOB) based on five taxonomic classification methods and quantification by qPCR (quantified abundances normalized against B16S). The numbers in sample codes denote experimental time in months. NA—not analyzed.
| SW0 | SW4 | SW8 | SWO4 | SWO8 | SWOB4 | SWOB8 | |
|---|---|---|---|---|---|---|---|
| Colwellia | |||||||
| Kaiju | 3.96 | 4.94 | 3.86 | 4.57 | 10.87 | 1.73 | 0.60 |
| Kaiju/MAR | 17.78 | 8.46 | 5.18 | 4.90 | 13.63 | 1.86 | 1.08 |
| Kraken2 | 8.32 | 9.97 | 6.26 | 4.94 | 10.83 | 1.40 | 0.61 |
| Bracken | 6.73 | 8.16 | 5.34 | 4.33 | 10.05 | 1.33 | 0.54 |
| Amplicon | 6.45 | 4.63 | 3.53 | 2.75 | 9.91 | 1.07 | 1.80 |
| Quantification | NA | NA | 7.35 | NA | 13.83 | 0.85 | 0.34 |
| Cycloclasticus | |||||||
| Kaiju | 0.13 | 10.05 | 8.81 | 1.10 | 2.12 | 1.82 | 13.36 |
| Kaiju/MAR | 0.25 | 23.20 | 16.69 | 1.75 | 3.99 | 2.66 | 25.33 |
| Kraken2 | 0.18 | 1.90 | 1.48 | 0.14 | 0.23 | 0.21 | 4.88 |
| Bracken | 0.14 | 1.55 | 1.25 | 0.12 | 0.21 | 0.20 | 4.34 |
| Amplicon | 0.05 | 2.13 | 1.90 | 0.25 | 0.46 | 0.59 | 7.74 |
| Quantification | NA | NA | 0.25 | NA | 0.01 | 0.14 | 7.60 |
| Pseudomonas | |||||||
| Kaiju | 1.96 | 1.23 | 1.97 | 9.64 | 6.42 | 38.41 | 25.39 |
| Kaiju/MAR | 0.48 | 0.94 | 2.24 | 8.59 | 9.44 | 39.03 | 13.88 |
| Kraken2 | 3.02 | 5.68 | 5.37 | 13.64 | 13.06 | 50.54 | 34.33 |
| Bracken | 2.71 | 4.74 | 4.83 | 12.21 | 11.97 | 48.14 | 31.65 |
| Amplicon | 0.26 | 0.20 | 0.25 | 0.78 | 0.24 | 10.76 | 3.70 |
| Quantification | NA | NA | 2.59 | NA | 5.08 | 9.52 | 9.25 |