Table 1.

Decellularization procedures of abdominoplasty skin.

Decellularization Protocol	Phase 1					Phase 2				Washing			Storage
	Reagents	Time (h)	Temp. (°C)	RPM	Epidermis Removal	Reagents	Time (h)	Temp. (°C)	RPM	Washing/Quantity	Time (h)	(RPM)	Lyophilization/Temp (°C)
ADM1	1M NaCl + antibiotic mix	24	37	40	Mechanical	SDS 0.1% + Antibiotic Mix	24	37	40	H2O/5	24	60	YES/−70
ADM2	2M NaCl	24	37	40	Mechanical	SDS 0.1% + Antibiotic Mix	24	37	40	H2O/5	24	60	YES/−70
ADM3	TrypLE Select + antibiotic mix	24	37	40	Not Applicable	3% Triton X-100 in PBS + Antibiotic Mix	24	37	40	H2O/5	24	60	YES/−70

NaCl—sodium chloride; SDS—sodium dodecyl sulfate; PBS—phosphate-buffered saline, H₂O—distilled deionized water, 5—five days washing step with daily H₂O change.