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. Author manuscript; available in PMC: 2022 Aug 5.
Published in final edited form as: Cell. 2021 Jul 7;184(16):4329–4347.e23. doi: 10.1016/j.cell.2021.06.023

Figure 2. Identification of neuron sub-types.

Figure 2.

A) UMAP of neurons with molecularly distinct subtypes (bold labels) from neuronal UMAP (Figure 1B). Inset denotes IL2 DV and IL2 LR clusters. B) Volcano plot of differentially expressed genes (FDR < 0.05) for ASER vs ASEL. Guanylyl cyclases (gcy), neuropeptides, and transcription factors are marked. C) (Top) 3 pairs of IL2 sensory neurons (IL2L/R, IL2VL/R, IL2DL/R) from WormAtlas. (Bottom) UMAP inset from A showing normalized expression of marker genes for all IL2 neurons (klp-6, unc-86), IL2 LR (unc-39, egas-4) and IL2 DV (egas-1). D) Volcano plot of differentially expressed genes (FDR < 0.05) between IL2 sub-types. E) (Top) VB motor neuron soma in the ventral nerve cord. (Bottom) sub-UMAPs of VB neurons highlighting VB marker (ceh-12) and genes (sptf-1, hlh-17, vab-23) expressed in specific VB sub-clusters. F) Confocal images in NeuroPAL show sptf-1::GFP expression in VB1 but not VB2 and G) selective expression of hlh-17::GFP in VB2 but not VB1. Scale bars = 10 μm. H) Volcano plot of differentially expressed genes (LGICs –ligand-gated ion channels) (FDR < 0.05) for VB1 vs all other VB neurons. I) C. elegans neuron types in a force-directed network by transcriptomic similarities. Colors denote distinct neuron modalities and widths of edges (Pearson correlation coefficients > 0.7) show strengths of transcriptome similarity between each pair of neuron types. See also Figure S4.