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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Nutrition. 2021 Feb 8;87-88:111189. doi: 10.1016/j.nut.2021.111189

Figure 6. NR increases both aerobic and anaerobic respiration in mature myoblasts.

Figure 6.

Schematic of how human derived myoblast were differentiated for 10 days into myotubes, while they were treated with 0.5 mM NR for the last 72 or 24 hours (A). The methodology explained in Figure 4 was used to measure aerobic respiration (B) and anaerobic respiration (C) using Seahorse extracellular flux analyzer. Data represents the mean of 4 to 5 wells per condition, with each experiment performed for three different donors to ensure repeatability. Statistical significance indicated by “*” p < 0.05, “**” p < 0.01, “***” p < 0.001, and “ns” indicating non-significance.