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. 2021 Nov 15;10:e68584. doi: 10.7554/eLife.68584

Figure 7. Regenerate scaling is not impacted by non-neural extrinsic factors in the neural-modified accessory limb model (NM-ALM).

Figure 7.

(A) Dorsal root ganglia (DRGs) from GFP+ donor animals (~14 cm) were grafted into wound sites on large (~14 cm, n = 12) and small (~7 cm, n = 19) host animals followed by mid-bud blastemas from (~7 cm) donor animals. (B) If non-neural extrinsic factors play an instructive role in size regulation, then the large host animals would produce a larger ectopic limb than those on the small host animal. If non-neural factors do not play an instructive role, then the ectopic limbs will be the same size, regardless of host size. (C) The ectopic limb lengths were the same size at 0 day post graft (DPG) and remained the same throughout regeneration (135 DPG). (D) The unamputated limbs on the control animals remained different sizes throughout the course of the experiment (n = 10). Error bars = SEM. p-Values calculated by t-tests. *** = p < 0.0005.