Genetic reagent (Ambystoma mexicanum) |
White-strain 518 |
Ambystoma Genetic Stock Center at the University of Kentucky |
RRID: AGSC_101J
|
|
Genetic reagent (Ambystoma mexicanum) |
GFP-strain |
Ambystoma Genetic Stock Center at the University of Kentucky |
RRID: AGSC_110J
|
|
Genetic reagent (Ambystoma mexicanum) |
RFP-strain |
Ambystoma Genetic Stock Center at the University of Kentucky |
RRID: AGSC_112J
|
|
Chemical compound, drug |
MS-222 |
Sigma-Aldrich |
E10521-50G |
|
Chemical compound, drug |
Formaldehyde |
RICCA Chemical Company |
R3190000-1A |
|
Other |
DAPI |
Sigma-Aldrich |
D9542-5MG |
(1:1000) dilution |
Chemical compound, drug |
Ethylenediaminetetraacetic acid (EDTA) |
VWR |
VWRV0105-500G |
|
Commercial assay or kit |
In Situ Cell Death Detection Kit, Fluorescein |
Roche |
11684795910 |
|
Commercial assay or kit |
Click-iT Plus EdU Proliferation Kit |
Roche |
C10337 |
100 ng EdU/Animal |
Software, algorithm |
ZenPro software |
Zeiss |
410138-1104-260 |
|
Software, algorithm |
FIJI ImageJ |
Open Source |
|
|
Other |
Tissue-Tek OCT Compound |
Sakura |
4583 |
|
Other |
Permount Mounting Medium |
Thermo Fisher Scientific |
SP15-100 |
|
Other |
VECTASHIELD612 Antifade Mounting Medium |
Vector Laboratories |
H-1000–10 |
|
Other |
Wheat-Germ Agglutinin |
Thermo FisherScientific |
W32466 |
|
Other |
Rhodamine phalloidin |
Thermo Fisher Scientific |
R415 |
|
Other |
Hematoxylin Solution, Harris Modified |
Sigma-Aldrich |
HHS16-500ml |
|
Other |
Eosin Y |
Thermo Fisher Scientific |
E511-100 |
|
Other |
Alcian Blue |
Sigma-Aldrich |
A-5268 |
|
Antibody |
(Mouse Monoclonal) Anti-Acetylated Tubulin antibody |
Sigma-Aldrich |
T7451-200UL |
(1:200) dilution |
Antibody |
(Goat polyclonal) Anti-Mouse IgG Alexa Fluor 488 |
Abcam |
ab150173 |
(1:200) dilution |