Figure 6.

BCS performance for protein-peptide binder system spot-tag

Spot-Tag binding performance counts demonstrated enrichment of nanobody-peptide binding on BCS. Experiments are run in replicates with different barcodes associated for each replicate. Difference in sequencing counts between experimental replicates is thought to be due to the difference in barcode used for each replicate. The impact of barcode sequence was screened and analyzed to derive a set of barcodes used for downstream experimentation. No known variables (GC content, sequential base pairs, etc.) were found to be related to a barcode's impact on sequencing noise outside of target type (DNA versus nanobody, etc.). Experiments were repeated and validated, confirming the protocol utilization for a DNA-DNA binding system and peptide-nanobody binding system. Table of counts is included in Table S8.