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. 2021 Dec 9;25(1):103588. doi: 10.1016/j.isci.2021.103588

Figure 1.

Figure 1

In vitro IL-27 stimulation leads to enhanced STAT1 activation in naive and memory phenotype T cells from PLWH

PBMCs from PLWH (HIV+, n = 12) and healthy controls (HC, n = 14) were thaw, rested overnight and stimulated with 25 ng/mL of rhIL-27, 100 U/mL of rhIFN⍺, and 100 ng/mL of rhIL-6, for 30 min. (A) Gating strategy of naive (CD45RA+CD27+) and memory (CD45RA−CD27+) phenotype CD4 and CD8 T cells. Median Fluorescence Intensity (MFI) of intracellular expression of t-STAT1 and frequency of phosphorylated STAT1 (p-STAT1) in: (B) naive and memory phenotype CD4 T cell subsets; (C) naive and memory phenotype CD8 T cell subsets. Median Fluorescence Intensity (MFI) of intracellular expression of t-STAT3 and frequency of phosphorylated STAT3 (p-STAT3) in: (D) naive and memory phenotype CD4 T cell subsets; and (E) naive and memory phenotype CD8 T cell subsets. The graph is represented by box and whisker showing the median value with first and third quartiles in the box, with whiskers extending to the minimum and maximum values. Comparisons between the study groups PLWH and healthy controls were performed using Mann–Whitney test. p value <0.05 was considered significant.