Figure 4.

Immunohistochemical analysis of 3D-bioprinted LAT explanted after 30 days in vivo. (a) The endothelial marker CD31 was used to visualize blood vessels apparent in the capsule and the centre of the construct (arrows). (b) Consecutive sections were stained with the anti-human specific marker Ku80. The blood vessels in the capsule were of mouse origin (Ku80⁻), whereas the vascular structures present in the centre of the constructs were human (Ku80⁺). The images were cropped and linearly adjusted for exposure and contrast. (c) Blood vessels with erythrocytes in the lumen were observed in 3D-bioprinted LAT constructs explanted after 150 days in vivo, indicating functioning blood vessels connected to mouse systemic circulation. The images were cropped and linearly adjusted for exposure and contrast.