Figure 7.

Validation of the linkage of AMPK-MTOR to Sphingolipid Metabolism in H₂O₂-activated human platelets. (A) Washed platelets (1 × 10⁷/mL) treated with 100 μM H₂O₂ for 60 min in the presence or absence of compound C (10 μM). After the reaction, sphingomyelin (SM) levels were determined by the ELISA assay. (B-D) Washed platelets (1 × 10⁶/mL) treated with 100 μM H₂O₂ for 60 min in the presence or absence of compound C (10 μM). After the reaction, the level of three sphingomyelins (B) SM 16:0 (d18:1/16:0); (C) SM 18:0 (d18:1/18:0); (D) SM 24:0 (d18:1/24:0) were quantified by the LC-MS/MS assay. Data (A-D) are presented as the means ± S.E.M. (n = 6). *P < 0.05, **P < 0.01, and ***P < 0.001, compared with the control group. ^#P < 0.05, compared with the H₂O₂-treated group