Skip to main content
. 2021 Jul 29;36(1):68–79. doi: 10.1038/s41375-021-01346-7

Fig. 3. Igf2bp3 is required for LIC function in endpoint colony formation assays.

Fig. 3

a Quantification of CD11b+c-Kit+ cells in the spleen of recipient mice at 14 weeks post transplantation (n = 4 Ctrl, n = 8 MLL-Af4; one-way ANOVA followed by Bonferroni’s multiple comparisons test; **P < 0.01). b Quantitation of CD11b+c-Kit+ cells in the BM 14 weeks post transplantation (n = 4 Ctrl, n = 8 MLL-Af4; one-way ANOVA followed by Bonferroni’s multiple comparisons test; **P < 0.01, ***P < 0.001). c Expression of IGF2BP3 of in WT/MLL-Af4 and I3KO/MLL-Af4 immortalized Lin− cells at the protein level. d Colony formation assay of WT/MLL-Af4 and I3KO/MLL-Af4 immortalized Lin− cells (t test; **P < 0.01). e Schematic of collection of Cas9-GFP MLL-Af4 Lin− cells and CRISPR-Cas9-mediated deletion of Igf2bp3. f Expression of Igf2bp3 in Cas9-GFP MLL-Af4 Lin− cells in nontargeting (NT) and Igf2bp3 deleted (I3sg) cells by RT-qPCR. g Expression of IGF2BP3 in NT and I3sg Cas9-GFP MLL-Af4 Lin− cells at the protein level. h Colony formation assay of NT and I3sg deleted Cas9-GFP MLL-Af4 Lin− cells (t test; *P < 0.05, **P < 0.01, ***P < 0.001).