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. 2021 Dec 9;12(1):320–331. doi: 10.1002/2211-5463.13340

The crystal structure of the FAM134B–GABARAP complex provides mechanistic insights into the selective binding of FAM134 to the GABARAP subfamily

Junfeng Zhao 1, Zhiwei Li 1, Jianchao Li 1,2,✉
PMCID: PMC8727931  PMID: 34854256

Abstract

The mammalian Atg8 family (Atg8s proteins) consists of two subfamilies: GABARAP and LC3. All members can bind to the LC3‐interacting region (LIR) or Atg8‐interacting motif and participate in multiple steps of autophagy. The endoplasmic reticulum (ER) autophagy receptor FAM134B contains an LIR motif that can bind to Atg8s, but whether it can differentially bind to the two subfamilies and, if so, the structural basis for this preference remains unknown. Here, we found that FAM134B bound to the GABARAP subfamily more strongly than to the LC3 subfamily. We then solved the crystal structure of the FAM134B–GABARAP complex and demonstrated that FAM134B used both its LIR core and the C‐terminal helix to bind to GABARAP. We further showed that these properties might be conserved in FAM134A or FAM134C. The structure also allowed us to identify the structural determinants for the binding selectivity. Our work may be valuable for studying the differential functions of GABARAP and LC3 subfamilies in ER phagy in future.

Keywords: Atg8, autophagy, FAM134B, GABARAP, LC3‐interacting region/Atg8‐interacting motif

The endoplasmic reticulum autophagy receptor FAM134B uses its Φ‐X1‐X2‐Ψ motif and C‐terminal α‐helix, or the “LC3‐interacting region core + C‐helix”, to bind to the GABARAP subfamily proteins preferentially and strongly.

graphic file with name FEB4-12-320-g005.jpg

Abbreviations

ER

endoplasmic reticulum

GIM

GABARAP‐interacting motif

ITC

isothermal titration calorimetry

K d

dissociation constant

LIR

LC3‐interacting region

PDB

protein data bank

SEC‐MALS

size‐exclusion chromatography coupled with multi‐angle light scattering

In eukaryotes, autophagy plays an important role in maintaining cellular homeostasis by degrading deleterious materials, such as protein aggregates, damaged organelles, and pathogens [1, 2, 3]. Autophagy is closely related to human diseases [4, 5]. Defects in autophagy may lead to neurodegenerative diseases, immune diseases, metabolic disorders, etc. [6, 7, 8, 9]. On the contrary, cancer cells can hijack autophagy pathways to provide sufficient nutrients and energy for their massive growth [10].

Autophagy is an evolutionarily conserved process involving multiple steps [2, 3, 11, 12, 13, 14]. It is controlled by a set of genes, including the Atg genes initially identified in yeast and EPG genes initially identified in Caenorhabditis elegans [15, 16]. Among them, Atg8 family proteins are engaged in nearly the entire process [17, 18, 19, 20]. For example, they can promote phagophore elongation and autophagosome formation [21, 22, 23]; they can help the fusion of autophagosome to lysosome [24, 25]; they can also recruit the autophagy receptors for selective target degradation [26, 27].

In mammals, Atg8 family contains two subfamilies, the GABARAP subfamily (including GABARAP, GABARAPL1, and GABARAPL2) and the LC3 subfamily (including LC3A, LC3B, and LC3C) [28]. They share high sequence similarity, and thus, they fold into similar three‐dimensional structures, that is, two N‐terminal α‐helix followed by a C‐terminal ubiquitin‐like domain [29, 30]. With these similar features, they can bind to proteins containing a motif called LC3‐interacting region (LIR) or Atg8‐interacting motif. This motif canonically contains a Φ‐X1‐X2‐Ψ motif, where Φ is an aromatic residue (W/Y/F), Ψ is an aliphatic residue (L/I/V), and X1/2 is any amino acid [31, 32]. These motifs normally bind to GABARAPs and LC3s with similar dissociation constants (K d) ranging from sub‐µm to a few µm. It was thought that these two subfamilies functioned redundantly in cells. However, more and more evidence shows that they play distinct roles in cells and are not interchangeable [23, 33, 34]. It is possible that some LIRs can differentiate these two subfamilies by binding to them with different affinities. Indeed, a study in 2017 showed that the Φ‐X1‐X2‐Ψ motif with a valine or isoleucine residue at the X1 position and a valine residue at the Ψ position ([W/F]‐[V/I]‐X2‐V, referred to as GABARAP‐interacting motif, GIM) preferably bound to GABARAPs 10‐ to 20‐fold stronger than to LC3s [35]. The discovery of LIR in giant ankyrin G (AnkG) revealed another possible mechanism [36, 37]. AnkG LIR uses an α‐helix (C‐helix) C‐terminal to the LIR core Φ‐X1‐X2‐Ψ motif to achieve extremely high affinity (a few nm) and high selectivity (it binds to GABARAPs 1000‐fold stronger than to LC3s). This “LIR core + C‐helix” mode has also been found in a series of LIR‐containing proteins including 440 kD ankyrin B, FYCO1, STX17, Ede1, Atg40, Sec62, RTN3, etc. [38, 39, 40, 41, 42]. Sequence‐based search has identified that FAM134B LIR might also fit this “LIR core + C‐helix” mode [37].

FAM134B, also called reticulophagy regulator 1, is a member of a family with sequence similarity 134. The family consists of three members, FAM134A/B/C, and studies on this family mainly focus on FAM134B. It can be anchored into the endoplasmic reticulum (ER) membrane via its transmembrane helices and bind to Atg8 family proteins via its LIR motif. Thus, it can serve as a selective autophagic receptor for ER autophagy (ER phagy) [43, 44, 45]. Dysfunction of FAM134B is associated with diseases such as inflammation, neuropathy, and cancer [46, 47, 48]. However, whether FAM134B LIR can preferentially bind to GABARAPs or LC3s and the structural basis for the preference remains elusive.

In this work, we studied the interactions between FAM134 family proteins and Atg8 family proteins in detail. We found that FAM134B preferred binding to the GABARAP subfamily, with about 10‐fold stronger affinity than to the LC3 subfamily. We then solved the crystal structure of FAM134B/GABARAP complex, revealing that FAM134B used the “LIR core + C‐helix” mode to bind to GABARAP and thus achieving the high affinity and high specificity. We also showed that the binding selectivity to GABARAP and the “LIR core + C‐helix” binding mode might be a general feature for the entire FAM134 family. Finally, our combined sequence and structural analyses provided insight into the structural determinants for the preferential binding. Our work might provide the biochemical foundation for further studying the differential functions of GABARAP and LC3 subfamilies in ER phagy.

Results

FAM134B_LIR interacts with GABARAP stronger than LC3A

Sequence analysis reveals that FAM134B LIR (aa 451–476) is conserved among species in vertebrates (Fig. 1A). A previous study showed that deleting its C‐terminal reduced the binding to GABARAP dramatically, indicating that it also conformed to the “LIR core + C‐helix” mode [37]. We first used size‐exclusion chromatography coupled with multi‐angle light scattering (SEC‐MALS) to characterize its interaction with GABARAPL1 (a close paralog of GABARAP with > 80% sequence identity and it behaved much better in the SEC column). When mixing the two recombinant proteins at a 1 : 1 molar ratio, they were eluted with smaller elution volume and the measured molecular weight of 31.5 kDa was close to its theoretical molecular weight of 33.3 kDa, indicating that they formed a tight complex (Fig. 1B). Consistently, the measured K d between FAM134B and GABARAP by isothermal titration calorimetry (ITC) was 42 nm (Fig. 1C), which was comparable to the previously reported 25 nm [37].

Fig. 1.

Fig. 1

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FAM134B LIR binds to GABARAP subfamily stronger than LC3 subfamily. (A) Sequence alignment showing that FAM134B LIR and its C‐terminal extension are highly conserved in vertebrates. The symbols above the sequences are defined as follows: an asterisk (*) indicates positions with a fully conserved residue; a colon (:) indicates conservation between groups of strongly similar properties; a period (.) indicates conservation between groups of weakly similar properties. Identical and highly similar residues are colored (yellow for hydrophobic residues, red for negatively charged residues, and green for other polar residues). (B, D) SEC‐MALS results (n = 3) showing that FAM134B strongly binds to GABARAPL1 (B) but binds to LC3B with lower affinity (D). (C, E) ITC‐based quantitative K d measurements (n = 2) showing that the binding affinity between FAM134B and GABARAP (C) is about 10‐fold stronger than that between FAM134B and LC3A (E).

We next wanted to know whether FAM134B LIR binds to LC3s stronger or weaker than GABARAPs. Surprisingly, SEC‐MALS results showed that although FAM134B LIR can still form a complex with LC3B, the measured molecular weight (26.2 kDa) of the complex deviated from the theoretical one (33.8 kDa), suggesting that the binding affinity might be lower than that between FAM134B LIR and GABARAPL1 (Fig. 1D). In line with this, ITC showed that FAM134B LIR bound to LC3A with K d around 376 nm (Fig. 1E), about 10‐fold weaker than that between FAM134B LIR and GABARAP.

Overall structure of FAM134B_LIR/GABARAP complex

To understand the molecular basis of how FAM134B LIR interacts with GABARAP and the structural determinants of the differential binding, we tried to determine the high‐resolution structure of the complex. Using the chemically synthesized FAM134B LIR peptide and the recombinant GABARAP protein for crystallization, rod‐like crystals were obtained, and they were diffracted up to 2.85 Å (Table 1). The structure was then solved by molecular replacement methods. Agreeing with our expectation, the aromatic and aliphatic residues within the LIR core of FAM134B inserts into the two canonical hydrophobic pockets of GABARAP and its C terminus forms an α‐helix to interact with α3 of GABARAP (Fig. 2A,B).

Table 1.

Statistics of X‐ray Crystallographic Data Collection and Model refinement. Numbers in parentheses represent the value for the highest resolution shell.

Data collection
Data sets FAM134B/GABARAP
Space group P3221
Wavelength (Å) 0.97915
Unit cell parameters (Å)

a = b = 73.250, c = 80.852

α = β=90°, γ = 120°
Resolution range (Å) 50–2.85 (2.90–2.85)
No. of unique reflections 6205 (310)
Redundancy 15.6 (15.9)
I/σ 19.6 (2.0)
Completeness (%) 99.9 (99.7)
R merge a (%) 7.2 (69.4)
CC1/2 (last resolution shell) b 0.970
Structure refinement
Resolution (Å) 50–2.85 (2.94–2.85)
R cryst c /R free d (%) 18.59/24.38 (36.09/39.94)
rmsd bonds (Å)/angles (°) 0.007/1.422
Average B factor (Å2) e 71.09
No. of atoms
Protein atoms 1142
Water 0
Ligands 0
No. of reflections
Working set 5818 (558)
Test set 365 (48)
Ramachandran plot regions e
Favored (%) 96.27
Allowed (%) 3.73
Outliers (%) 0
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a

R merge = S |Ii  − <I>|/SIi , where Ii is the intensity of measured reflection and <I> is the mean intensity of all symmetry‐related reflections.

b

CC1/2 were defined by Karplus and Diederichs [63].

c

R cryst = Σ||F calc| − |F obs||/ΣF obs, where F obs and F calc are observed and calculated structure factors.

d

R free = Σ T ||F calc| − |F obs||/ΣF obs, where T is a test data set of about 5% of the total unique reflections randomly chosen and set aside prior to refinement.

e

B factors and Ramachandran plot statistics are calculated using MOLPROBITY [62].

Fig. 2.

Fig. 2

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Overall structure of FAM134B/GABARAP complex. (A) Ribbon representations of the FAM134B/GABARAP complex crystal structure. The FAM134B is colored orange, and GABARAP is colored light blue. This coloring scheme is used throughout the whole paper except as otherwise indicated. (B) A combined surface (GABARAP) and ribbon/stick (FAM134B) representation showing the two residues at the Φ site and the Ψ site insert into the hydrophobic pockets of GABARAP. (C) Superposition of our solved structure and the recently reported FAM134B/GABARAP (FAM134B in gold and GABARAP in pale cyan) structure using fusion strategy (PDB: 7BRQ).

During the period of our study, we noticed that a structure of FAM134B/GABARAP complex has been reported [41]. Comparing our structure to theirs, we found a few differences. First of all, the crystallization strategies were different. The crystallization for our structure determination was facilitated by mixing FAM134B peptide and GABARAP protein, whereas their structure was obtained by fusing FAM134B to the N terminus of GABARAP for successful crystallization. Moreover, the boundary of FAM134B we used for our structural study was aa 451–476 whereas they used aa 450–468. We believed that the longer C terminus and the direct mixing instead of fusion of the two proteins might better reflect the real state of how FAM134B C‐helix interact GABARAP, even though the resolution of our structure was lower. Indeed, by superimposing the two structures together, we can see that FAM134B C‐helix in our structure is a three‐turn helix and it turns inward and downward compared with their structure (Fig. 2C). The different conformations exhibited by the two structures might be due to the longer C‐helix and no steric hindrance induced by fusion of another GABARAP molecule to its C terminus.

Detailed interactions between FAM134B_LIR and GABARAP

In addition to the insertion of F455 and L458 into the two hydrophobic pockets of GABARAP, the other residues in the LIR core of FAM134B also interact with GABARAP. Although FAM134B shows specificity to GABARAPs, it does not fit the pattern of the previously reported GIM [35]. The residue following the “Φ” site is not a Val or Ile but a Glu. This E456 has charge–charge interaction with R67 of GABARAP (Fig. 3A). Mutating E456 to a positively charged Arg severely impacted the binding, as shown by our SEC‐MALS and GST pull‐down experiments using purified proteins (Fig. 3C–E). L457, another residue flanked by the “Φ” site and “Ψ” site, also makes hydrophobic contact with Y25 of GABARAP (Fig. 3A).

Fig. 3.

Fig. 3

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Detailed interactions between FAM134B and GABARAP. (A) E456 and L457 at the X1 and X2 sites are critical for FAM134B to bind to GABARAP as shown in the combined ribbon and stick representations. (B) The C‐helix of FAM134B is extensively involved in the interactions between FAM134B and GABARAP. (C) SEC results (n = 3) showing that the E456R mutation severely impaired the binding and L463Q mutation weakened the binding. (D) Representative pull‐down experiments (n = 4) showing that mutating critical residues in FAM134B weakened its interaction with GABARAP. (E) Quantification of the relative amount of GABARAP pulled down in the assays shown in panel D. The data were derived from four different batches of experiments, and the error bars were expressed as mean ± SEM and were analyzed with graphpad prism 9 using one‐way ANOVA followed by Tukey's multiple comparisons test; **P < 0.01; ***P < 0.001.

The C‐helix of FAM134B is also extensively involved in the binding (Fig. 3B). D459 and Q460 proceeding the C‐helix form salt bridge and hydrogen bond with R28 and D54, respectively. E462 at the beginning of the C‐helix forms a salt bridge with R67 of GABARAP. Notably, this salt bridge has been observed at several other C‐helix containing LIR/Atg8s complexes, for example, AnkG/B and FYCO1 [37, 39]. Our structural observation was consistent with the previous mutagenesis result and our current GST pull‐down assay (Fig. 3D,E) that E462R mutation dramatically impaired FAM134B's binding to GABARAP. This salt bridge might be important for anchoring the C‐helix at the right position. FAM134B C‐helix is an amphiphilic helix, like the C‐helix of AnkG/B LIR. The hydrophobic side consisting of L463 and I466 forms hydrophobic interaction with F62 and L63, while the hydrophilic residue E467 forms a hydrogen bond with Q59. Again, the SEC‐MALS result showed that mutating L463 to a polar residue Gln mildly weakened the interaction (Fig. 3C). GST pull‐down assay also showed that slightly less amount of GABARAP was pulled down using GST‐FAM134B L463Q or I466Q mutant, compared with WT. In sum, our structure revealed how FAM134B used its LIR core and C‐helix to interact with GABARAP.

FAM134B belongs to the family with sequence similarity 134. The family contains another two members, FAM134A and FAM134C. Similarly, they can also use their LIRs to interact with both GABARAPs and LC3s [43]. Sequence alignment showed that both the LIR and the residues following in FAM134 family are highly conserved (Fig. S1A), suggesting that FAM134A and FAM134C might also conform to the “LIR core + C‐helix” mode. FAM134A and FAM134C likely exhibited similar binding selectivity to GABARAPs. To test our hypothesis, we first used SEC to qualitatively study the binding between FAM134A and Atg8s. When mixing FAM134A and GABARAPL1 at a 1 : 1 molar ratio, these two proteins were co‐eluted at a smaller elution volume, indicating that they interacted with each other. In contrast, when mixing at a 1 : 1 molar ratio, only a small portion of LC3B was co‐eluted with FAM134A and the majority of LC3B was eluted as a separated peak, suggesting that FAM134A bound to LC3B weakly (Fig. S1B,C). We failed to use ITC to quantitatively determine the K d values for the bindings for direct comparison as the heat generation when titrating FAM134A to GABARAP/GABARAPL1 was too small to give reliable measurements (data not shown).

Structural basis for the preferential binding between FAM134B and GABARAP

We next wondered whether our structure can provide some implications of the reasons why FAM134B binds to GABARAPs stronger than LC3s. GABARAPs and LC3s originate from the same ancestor Atg8 and are structurally similar, but the two subfamilies evolved separately since the appearance of metazoan. As a result, the sequence identities among the same subfamily were over 80%, whereas the sequence identities between GABARAP/GABARAPL1 and LC3A/B were only 30–35%, as revealed by our analyses on human Atg8s sequences (Fig. 4A and Table S1). We anticipated that the structural determinants for the preferential binding to GABARAP might be the sites that were conserved in GABARAPs but not in LC3s.

Fig. 4.

Fig. 4

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Combined sequence and structural analysis of the FAM134B/GABARAP binding selectivity. (A) Sequence alignment of human Atg8 family proteins. Residues that are identical and highly similar are shaded in blue and light blue, respectively. The secondary structure elements derived from the GABARAP structure are shown at the top and those from the LC3A structure are shown at the bottom of the alignment. Residues that might be critical for selectivity are highlighted in orange boxes. (B–E) Superposition of the FAM134B/GABARAP and the FAM134B/LC3B (light orange for FAM134B and pale cyan for LC3B) structure showing a few differences in the binding details. The F455 and L458 at the Φ site and the Ψ site are also shown to indicate the relative positions. (F) Representative competitive pull‐down experiments (n = 3) showing that the relative pulled‐down amount of GABARAP and LC3A were affected by exchange mutations of critical residues. (G) Quantification of the relative amount of GABARAP and LC3A pulled down in the assays shown in panel F. The data are derived from three different batches of experiments, and the error bars are expressed as mean ± SEM and were analyzed with graphpad prism 9 using one‐way ANOVA followed by Tukey's multiple comparisons test; ns: not significant, P > 0.05 **P < 0.01; ***P < 0.001.

We aligned the previously reported FAM134B/LC3A and our currently solved FAM134B/GABARAP structure together (Fig. 4B–E). By combining the sequence alignment of human Atg8 family proteins, we indeed found a few differences. First, Y25 of GABARAP forms a hydrogen bond with D453 of FAM134B. The corresponding residue in LC3A/B is a His and in LC3C is a Phe. In the FAM134B/LC3A structure, the H27 in LC3A does not interact with D453, as this residue points in the opposite direction (Fig. 4A,B). Moreover, D459, following the LIR core motif, forms a salt bridge with R28 in FAM134B/GABARAP structure. Although the corresponding residue of R28 in LC3s is also a positively charged residue Lys, it does not interact with D459 as shown in the FAM134B/LC3A structure (Fig. 4A,C). Furthermore, D54 of GABARAP forms a hydrogen bond with Q460. Q460 is not present in the FAM134B/LC3A structure, but it can be seen from the structure that H57, the counterpart of D54 in LC3A, is closer to the position of Q460. This might cause steric hindrance and weaken its binding to FAM134B (Fig. 4A,D). Finally, F62, located at GABARAP α3, makes hydrophobic contact with the amphiphilic C‐helix (Fig. 4A,E). It has already been shown that this Phe plays a critical role in dictating the binding selectivity between AnkG LIR and GABARAPs. It might also be important for FAM134B's preferential binding.

We tried to use GST pull‐down assay with purified recombinant proteins to verify the above observation. Agreeing with our analysis, mutating the four sites in LC3A to the corresponding residues of GABARAP (i.e., H27Y, K30R, H57D, and K65F) all enhanced the binding to GST‐FAM134B (Fig. S2). Then, we performed competition experiments to see whether the GABARAP/LC3A exchanged mutants can change the selectivity. For the WT group, the GST‐FAM134B could pull down more GABARAP and less LC3A (Fig. 4F), which is consistent with other biochemical results that FAM134B bound to GABARAP stronger than LC3A (Fig. 1). In two of the exchange mutations (H27Y/Y25H and K30R/R28K) groups, we could see more LC3A mutant and less GABARAP mutant being pulled down, compared with the WT group (Fig. 4F,G). For the H57D/D54H and K65F/F62K groups, we could not see more LC3A being pulled down in the presence of the GABARAP mutant (Fig. 4F,G). The reason might be that GABARAP carrying these two mutations also increased the binding to FAM134B. Nevertheless, the above pull‐down experiments verified that the four sites we identified prevent LC3A from strongly binding to FAM134B and thus contribute to the binding selectivity.

General structural features of the “LIR core + C‐helix” binding mode

More and more high‐resolution structures of the “LIR core + C‐helix” mode have now become available. Here, we try to summarize some general features revealed by these structures. We chose our currently solved FAM134B/GABARAP, and the previously reported AnkG/GABARAPL1, AnkB/GABARAP, STX17/GABARAP, RTN3/GABARAP, SEC62/GABARAP, FYCO1/LC3A, Ede1/Atg8 structures for detailed analysis [37, 39, 40, 41, 42]. By aligning all these structures together, we can see that these C‐helices vary in numbers of turns and orientations (Fig. 5). Despite these differences, there are a few common features. Firstly, except for RTN3, all these helices anchor to α3 of Atg8s via a salt bridge formed by an Arg (R67 in GABARAP/GABARAPL1, R70 in LC3A, and R70 in yeast Atg8) from Atg8s and a Glu or Asp (E462 in FAM134B, E1996 in AnkG, E1599 in AnkB, D178 in STX17, E370 in SEC62, E1287 in FYCO1, and D1245 in Ede1) from LIR‐containing proteins. In addition to the salt bridge, all C‐helices use a hydrophobic residue at essentially the same spatial position (L463 in FAM134B, A2000 in AnkG, A1603 in AnkB, L182 in STX17, F257 in RTN3, L371 in Sec62, I1291 in FYCO1, and L1246 in Ede1) to form hydrophobic interaction with α3 of Atg8s. The positions of the Glu or Asp in the primary sequences are mainly 4 residues C‐terminal to the Ψ site, that is, the +7 position (Fig. S3). The exceptional case is STX17, the Asp in which is only three residues apart, that is, the +6 position (Fig. S3). The relative positions between the hydrophobic residues and the acidic residues in the primary sequence can be grouped into two categories. In one category (FAM134B, SEC62, and Ede1), the two residues are precisely next to each other (+7 & +8 positions, Fig. S3), while in the other category (AnkG, AnkB, FYCO1, and STX17; although the +8 positions are also hydrophobic residues, they do not interact with α3 of Atg8s, Fig. 5E–H), the two residues are 4 residues apart (+7 & +11 positions, two neighboring turns in the three‐dimensional structures, Fig. S3). This variation may be due to the different orientations of the C‐helices. This explains why the consensus sequence derived from AnkG/B and FYCO1 failed to predict the LIRs in the other category. Nevertheless, these two common interactions together with other specific interactions facilitate the C‐helices binding.

Fig. 5.

Fig. 5

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Summary of LIRs/Atg8s structures conforming to the “LIR core + C‐helix” binding mode. (A–H) Eight LIRs/Atg8s complex structures are selected and aligned together: our FAM134B/GABARAP (panel A), the previously reported SEC62/GABARAP (panel B), Ede1/Atg8 (panel C), RTN3/GABARAP (panel D), FYCO1/LC3A (panel E), AnkG/GABARAPL1 (panel F), AnkB/GABARAP (panel G), and STX17/GABARAP (panel H) structures. The sequences of these LIRs are also shown to indicate the relative positions of the Φ site and the Ψ site (highlighted in brown) in the LIR cores and the acid residue (highlighted in red) and hydrophobic residue (highlighted in orange) in the C‐helices.

It is worth noting that either of these two interactions can be missing if there are some compensatory interactions. For example, the C‐helix of RTN3 does not form a salt bridge with R67 of GABARAP. Instead, the hydrophobic interaction made by the two bulky residues F257 and F261 facilitates the anchoring of the C‐helix. Meanwhile, some LIRs with C‐terminal extensions also contain one or both of these elements. In the Atg40/Atg8 complex structure, D247 of Atg40 forms a salt bridge with R67 of Atg8. In the GABAA receptor γ2 subunit (γ2‐GABAAR)/GABARAPL1 complex structure, the C413 of γ2‐GABAAR occupies the same position of the hydrophobic residues of other C‐helix containing LIRs [49].

Discussion

Atg8 family proteins are central players throughout the entire autophagic process. To fulfill the diverse functions, they are able to bind to various proteins with the Φ‐X1‐X2‐Ψ motif. Growing evidence has shown that the GABARAP subfamily and the LC3 subfamily, or even each individual member, function distinctly in some specific process. Consistently, some of the LIR‐containing proteins, including but not limited to AnkG, FYCO1, PLEKHM1, PCM1, ULK1, γ2‐GABAAR, and FAM134 family proteins in this study, can differentially bind to the two subfamilies [35, 37, 39, 49, 50]. These proteins can be vaguely classified into two subgroups: one conforms to the previously identified GIM or [W/F]‐[V/I]‐X2‐V motif [35], and the other contains C‐terminal extension following the LIR core motif. We demonstrated here that FAM134 family belongs to the second group and the C‐terminal extension forms an α‐helix.

The “LIR core + C‐helix” binding mode was originally recognized in FYCO1 and AnkG and later found in a lot more LIR‐containing proteins, such as 440 kD ankyrin B, STX17, Ede1, Atg40, SEC62, RTN3, etc. The C‐helix mainly functions in two aspects. Firstly, it can greatly enhance the binding between LIR‐containing proteins and Atg8 family proteins. Take FAM134B in this study as an example. The involvement of the C‐helix enhances the binding affinity by more than 10‐fold. A similar enhancement effect was also found in AnkG/B [37]. Secondly, the C‐helix may contribute to the selective binding to GABARAPs or LC3s. The differences in hydrophobicity of Atg8s α3 have been noticed in an earlier study by Wirth et al. [50]. It is shown in our FAM134B/GABARAP and the previously solved AnkG/Atg8s structures that the C‐helices are amphiphilic, with their hydrophobic sides facing the α3. This feature allows the selection of different C‐helix containing LIR for strong binding.

Our FAM134B/GABARAP structure, along with other C‐helix containing LIR structures, reveals some common features of the C‐helices binding, but at the same time also highlights the diversities of the C‐helices. This implies the target recognition mechanisms of Atg8s are far beyond the current knowledge on the Φ‐X1‐X2‐Ψ motif. It is highly possible that residues N‐terminal or C‐terminal to the core motif contribute to the Atg8s/targets recognition with high affinity and high specificity. This also brings out new challenges on how to predict such targets.

The structure of FAM134B/GABARAP also underlines the importance of R67 in GABARAP (or the corresponding R70 in LC3A, R67 in yeast Atg8). On the one hand, as discussed above, R67 is responsible for making a salt bridge with acidic residues in C‐helices for their anchoring. On the other hand, R67 can also form a salt bridge with the acidic residue (E456 in FAM134B) at the X1 position of the Φ‐X1‐X2‐Ψ motif. Indeed, sequence analysis showed that nearly half of the LIRs contain an acidic residue at the X1 position. By inspecting some of the reported structures (e.g., RTN3/GABARAP, SEC62/GABARAP, STX17/GABARAP, Calreticulin/GABARAP, ATG14/GABARAPL1, ATG4B/GABARAPL1, γ2‐GABAAR/GABARAPL1, FYCO1/LC3A, FUNDC1/LC3B, TECPR2/LC3B, NEDD4/LC3B, Atg40/Atg8, Ede1/Atg8), we find that all the acidic residues, no matter Glu or Asp, invariably interact with the Arg in Atg8s [40, 41, 42, 49, 51, 52, 53, 54, 55, 56].

Last but not least, in contrast to the earlier study, we showed using our biochemical approaches (combination of ITC, SEC‐MALS, and GST pull‐down assays) in this study that FAM134 family proteins bound to GABARAPs stronger than to LC3s. Similarly, another ER‐phagy receptor, ATL3, has been reported to specifically bind to GABARAPs but not LC3s recently [57]. The differential binding to GABARAPs or LC3s by ER‐phagy receptors implies that these two subfamilies may have their distinct and specific functions in ER phagy. Further follow‐up investigations will help to address this remaining question.

Conclusions

In summary, our biochemical and structural analysis demonstrated that FAM134B used the Φ‐X1‐X2‐Ψ motif and C‐terminal α‐helix, that is, the LIR core + C‐helix mode, to bind to the GABARAP subfamily proteins preferentially and strongly.

Materials and methods

Constructs, protein expression, and purification

The coding sequence of the human FAM134A fragment (Uniprot: Q8NC44, residues 488–513) was PCR‐amplified from synthetic oligonucleotides. The coding sequences of FAM134B (Uniprot: Q9H6L5), GABARAP (UniProt: Q9DCD6), GABARAPL1 (UniProt: Q8R3R8), LC3A (UniProt: Q91VR7), LC3B (UniProt: Q9CQV6) were generous gifts from M. Zhang (The Hong Kong University of Science and Technology). FAM134B peptide (residues 451–476) was synthesized from Shenzhen Pepbiotic Co. Ltd, Shenzhen, China. All constructs used for protein expression were cloned into pGEX‐6P‐1 or home‐modified pET32a vector. All point mutations were created using the QuikChange site‐directed mutagenesis kit and confirmed by DNA sequencing. Recombinant proteins were expressed in BL21 (DE3) Escherichia coli cells with induction by 0.1 mm IPTG at 16 °C. The N‐terminal Trx‐His6‐tagged proteins were purified using a Ni2+‐NTA agarose affinity column and GST‐tagged proteins were purified using Glutathione Sepharose, followed by size‐exclusion chromatography (Superdex 75 or Superdex 200 column from Cytiva, Marlborough, MA, USA) in a final buffer containing 100 mm NaCl, 50 mm Tris/HCl (pH 7.5), 1 mm DTT, and 1 mm EDTA. The purities and molecular weights were verified by SDS/PAGE and SEC‐MALS.

Crystallography

The crystals of FAM134B/GABARAP were grown in a solution containing 0.1 m bis‐tris propane pH 8.0, 2.2 m dl‐Malic acid by hanging drop vapor diffusion methods at 16 °C. Crystals were soaked in the mother liquor containing additional 20% glycerol for cryoprotection before diffraction experiments. The diffraction data were collected at the wavelength of 0.97915 Å and temperature of 100 K at the Shanghai Synchrotron Radiation Facility BL18U1 beamline. Data were further processed and scaled using hkl3000 software [58].

The structure was solved by phaser software [59] using molecular replacement method with the apo‐form structure of GABARAP [protein data bank (PDB): 5YIR] as the search model. The model of FAM134B peptide was manually built according to the difference electron‐density map in coot [60]. Further model modifications and refinements were repeated alternatively using coot software and REFMAC5 software [61]. The final model was validated using MolProbity [62], and the statistics are shown in Table 1. The structure figures were made using pymol software (https://pymol.org/2/).

Size‐exclusion chromatography coupled with multi‐angle light scattering

SEC‐MALS assays were performed on an ÄKTA Pure system (Cytiva) coupled with a Superose™ 12 10/300GL column, a static light scattering detector (miniDawn; Wyatt Technology), and a differential refractive index detector (Optilab; Wyatt Technology, Santa Barbara, CA, USA) in a column buffer composed of 100 mm NaCl, 50 mm Tris/HCl (pH 7.5), 1 mm DTT and 1 mm EDTA. Data were analyzed using ASTRA 7.3.1 (Wyatt Technology).

Isothermal titration calorimetry

ITC experiments were performed on a VP‐ITC Microcal calorimeter (Malvern) at 25 °C. Titration buffer contained 50 mm Tris/HCl (pH 7.5), 100 mm NaCl, 1 mm DTT, and 1 mm EDTA. For a typical experiment, each titration point was obtained by injecting a 10 μL aliquot of GABARAP or LC3A sample (350 μm) into the cell containing Trx‐tagged FAM134B (35 μm) at a time interval of 120 s to ensure that the titration peak returned to the baseline. The titration data were analyzed using the one‐site binding model by Origin7.0.

GST pull‐down assays

For GST pull‐down assays of FAM134B mutants or LC3A mutants, 1 µm GST‐tagged FAM134B or equimolar GST‐tagged FAM134B mutants (or GST as the negative control) was first incubated with the purified proteins of His‐tagged GABARAP or MBP‐tagged LC3A (or its mutants) for 1 h at 4 °C. For the competition experiments, 4 µm GST‐tagged FAM134B was first incubated with the purified proteins of 4.8 µm His‐tagged GABARAP (or its mutants) and 4.8 µm MBP‐tagged LC3A (or its mutants) for 1 h at 4 °C. Then, the 30 µL GSH‐Sepharose 4B slurry beads in PBS buffer were then incubated with the mixture for 30 min at 4 °C. After three times washing, the captured proteins were eluted by boiling, resolved by 10% SDS/PAGE, and detected by Coomassie blue staining. The Coomassie Brilliant Blue‐stained gels were analyzed by densitometry to determine the amount of pulled‐down proteins. Each experiment was repeated three times and data were analyzed with graphpad prism 9 (Graphpad Software, Inc., La Jolla, CA, USA) using one‐way ANOVA followed by Tukey's multiple comparisons test.

Conflict of interest

The authors declare no conflict of interest.

Author contributions

JZ and ZL performed experiments; all authors analyzed data; JL wrote the paper with inputs from other authors; and JL coordinated the project.

Supporting information

Fig. S1. FAM134A binds to GABARAP subfamily stronger than to LC3 subfamily.

Fig. S2. FAM134B binds to LC3A mutants stronger than its WT.

Fig. S3. Sequence alignment of C‐helix containing LIRs shown in Fig. 5.

Table S1. Sequence identities among human Atg8 family proteins

Click here for additional data file. (943KB, docx)

Acknowledgements

We thank the Shanghai Synchrotron Radiation Facility (SSRF) beamlines BL18U1 for X‐ray beam time. We also thank Dr. Ruichi Zhu for initiating the project. The constructs of Atg8s and FAM134B were generous gifts from Prof. Mingjie Zhang, the Hong Kong University of Science and Technology. This work was supported by grants from the National Natural Science Foundation of China (no. 21907033 and 31971140) to J.L.

Zhao J, Li Z, Li J. The crystal structure of the FAM134B–GABARAP complex provides mechanistic insights into the selective binding of FAM134 to the GABARAP subfamily. FEBS Open Bio.2022;12:320–331. 10.1002/2211-5463.13340

Edited by Alex Wlodawer

Data accessibility

The atomic coordinates and the structure factors of FAM134B/GABARAP complex structure are available from the PDB under the accession code 7FB5.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Fig. S1. FAM134A binds to GABARAP subfamily stronger than to LC3 subfamily.

Fig. S2. FAM134B binds to LC3A mutants stronger than its WT.

Fig. S3. Sequence alignment of C‐helix containing LIRs shown in Fig. 5.

Table S1. Sequence identities among human Atg8 family proteins

Click here for additional data file. (943KB, docx)

Data Availability Statement

The atomic coordinates and the structure factors of FAM134B/GABARAP complex structure are available from the PDB under the accession code 7FB5.

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